{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:17:21Z","timestamp":1740104241062,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has been proved that the expression of TLR4 is associated with a reduced risk of osteoporosis (OP). One single\u2010nucleotide polymorphism located within the 3\u2032\u2010untranslated region (3\u2032\u2010UTR) of TLR4 may \u201cgenerate\u201d binding site of miR\u201034a and thereby associated with risk of OP. Bioinformatics analysis and luciferase reporter assay were used to specify the effect of polymorphisms on the interaction between miR\u201034a and TLR4 gene. Western blot analysis and real\u2010time polymerase chain reaction were used to study the expressions of miR\u201034a, TLR4 in different groups or cells transfected with miR\u201034a mimics or inhibitor. 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide assay was used to estimate the effect of miR\u201034a on the apoptosis of osteoblast. TLR4 was identified as a target of miR\u201034a, with negative regulatory relationship predicted. The expression levels of miR\u201034a was comparable with each other between CC, CA, and AA groups, and the expression levels of TLR4 was evidently lower in CC compared with GG and GC groups. Also, TLR4 level in culture osteoblast (genotyped as CC) treated with miR\u201034a mimics was substantially downregulated compared with scramble control, while those cells (genotyped as CC) treated with miR\u201034a inhibitors showed increased expression of TLR4. Additionally, the apoptosis of osteoblast genotyped as CC was decreased following transfection with miR\u201034a mimics, while evidently promoted subsequent to transfect with miR\u201034a inhibitor. The regulatory association between rs1057317 polymorphism in TLR4 3\u2032\u2010UTR led to an inhibitory effect on the expression of TLR4 by miR\u201034a, which may explain the observed association between the polymorphism and the susceptibility to OP.<\/jats:p>","DOI":"10.1002\/jcb.28649","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T06:28:19Z","timestamp":1555396099000},"page":"13765-13774","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Identification of association between rs1057317 polymorphism in TLR4 3\u2032\u2010untranslated region and the susceptibility to osteoporosis"],"prefix":"10.1002","volume":"120","author":[{"given":"Ronghua","family":"Hong","sequence":"first","affiliation":[{"name":"Orthopedics Department Taizhou First People's Hospital Taizhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Xie","sequence":"additional","affiliation":[{"name":"Oncology Department Taizhou Hospital of Zhejiang Province Taizhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuguo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Orthopedics Department Taizhou First People's Hospital Taizhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hansong","family":"Pan","sequence":"additional","affiliation":[{"name":"Orthopedics Department Taizhou First People's Hospital Taizhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3868-3575","authenticated-orcid":false,"given":"Changjun","family":"Guo","sequence":"additional","affiliation":[{"name":"Orthopedics Department Taizhou First People's Hospital Taizhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130203"},{"key":"e_1_2_8_1_3_1","first-page":"1","article-title":"Assessment of fracture risk and its application to screening for postmenopausal osteoporosis. 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