{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:19:39Z","timestamp":1770819579792,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T00:00:00Z","timestamp":1554249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neonatal hypoxic\u2010ischemic encephalopathy is one of the leading causes of death in infants. Increasing evidence indicates that oxidative stress and apoptosis are major contributors to hypoxic\u2010ischemic injury and can be used as particularly promising therapeutic targets. Platycodin D (PLD) is a triterpenoid saponin that exhibits antioxidant properties. The aim of this study was to evaluate the effects of PLD on hypoxic\u2010ischemic injury in primary cortical neurons. We found that oxygen\u2010glucose deprivation\/reperfusion (OGD\/R) induced inhibition of cell viability and cytotoxicity, which were attenuated by PLD treatment. PLD treatment inhibited oxidative stress induced by OGD\/R, which was evidenced by the reduced level of reactive oxygen species and increased activities of catalase, superoxide dismutase, and glutathione peroxidase. Histone\u2010DNA enzyme\u2010linked immunosorbent assay revealed that apoptosis was significantly decreased after PLD treatment in OGD\/R\u2010treated cortical neurons. The increased bax expression and decreased bcl\u20102 expression induced by OGD\/R were reversed by PLD treatment. Furthermore, PLD treatment caused the activation of the phosphatidylinositol 3\u2010kinase (PI3K)\/protein kinase B (Akt)\/mammalian target of rapamycin (mTOR) pathway in OGD\/R\u2010stimulated cortical neurons. Suppression of this pathway blocked the protective effects of PLD on OGD\/R\u2010induced cell injury. These findings suggested that PLD executes its protective effects on OGD\/R\u2010induced cell injury via regulating the PI3K\/Akt\/mTOR pathway in cortical neurons.<\/jats:p>","DOI":"10.1002\/jcb.28677","type":"journal-article","created":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T06:59:48Z","timestamp":1554361188000},"page":"14028-14034","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Platycodin D protects cortical neurons against oxygen\u2010glucose deprivation\/reperfusion in neonatal hypoxic\u2010ischemic encephalopathy"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2203-0222","authenticated-orcid":false,"given":"Guifang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatrics Xinxiang Central Hospital Xinxiang Henan 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