{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T22:15:08Z","timestamp":1783116908566,"version":"3.54.6"},"reference-count":55,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673074"],"award-info":[{"award-number":["81673074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Alopecia areata (AA) is a common inflammatory disease characterized by cellular infiltration of T cells targeting the anagen\u2010stage hair follicle. Lack of efficacious treatment for AA may be due to little knowledge about its exact cellular mechanism. Studies have demonstrated that microRNAs (miRNAs) play an important role in the regulation of inflammatory skin diseases such as atopic dermatitis and psoriasis. However, little is known about the role of miRNAs in AA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The present study aimed to explore the blood miRNAs alterations in patients with severe active AA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We constructed a bipartite miRNA\u2010messenger RNA (mRNA) regulatory network by the validated\u00a0miRNA\u2010mRNA relationships. Subsequently, the miRNA\u2010miRNA synergistic network was formed in consideration of the Gene Ontology function enrichment of coregulated target genes. Lastly, the functional network was identified by the ingenuity pathway analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>By using an Agilent microarray that covers 2549 human miRNAs, we identified 36 significantly differentially expressed miRNAs in severe active AA patients. miRNA target gene prediction and functional annotation analysis showed significant enrichment in several pathways including the ribosome, cancer, cell cycle, insulin signaling, transforming growth factor\u2010\u03b2signaling, and p53 signaling pathways. Analysis of the three kinds of network showed that miR\u2010185\u20105p, miR\u2010125b\u20105p, and miR\u2010186\u20105p might play important and synergistic roles in the active phase of AA. According to the receiver operating characteristic curve analysis, several miRNAs were selected for the quantitative real\u2010time polymerase chain reaction validation. Among the miRNAs, miR\u2010210 and miR\u20101246 had high prediction with high accuracy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Blood dysregulated miRNAs are potentially associated with the severe active AA. These miRNAs could function synergistically and might be promising targets for the development of novel treatments for AA in the future.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.28700","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T07:12:45Z","timestamp":1555312365000},"page":"14421-14430","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Identification of blood microRNA alterations in patients with severe active alopecia areata"],"prefix":"10.1002","volume":"120","author":[{"given":"Youyu","family":"Sheng","sequence":"first","affiliation":[{"name":"Department of Dermatology Huashan Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sisi","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Huashan Hospital Fudan University Shanghai 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