{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:16:25Z","timestamp":1788232585856,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["IRIS ID: 2014\u20100099"],"award-info":[{"award-number":["IRIS ID: 2014\u20100099"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The emergence of multidrug resistance (MDR), extensively drug\u2010resistant, and total drug\u2010resistant <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic>Mtb<\/jats:italic>) strains have hampered the treatment of tuberculosis (TB). Capreomycin and Bedaquiline are currently used for MDR\u2010TB treatment. To understand the impact of these antibiotics on <jats:italic>Mtb<\/jats:italic> genes, we have curated the gene expression data where the <jats:italic>Mtb<\/jats:italic> cultures were exposed to the Bedaquiline and Capreomycin. Based on the <jats:italic>P<\/jats:italic> value cut off (&lt;0.05) and logFC (&lt;\u22120.5 and &gt;+0.5) values, we have selected the top differentially expressed genes during the antibiotic exposures. We have observed that the top differentially expressed <jats:italic>Mtb<\/jats:italic> genes were related to universal stress genes, two\u2010component regulatory systems, and drug efflux pumps. We have curated the <jats:italic>Mtb<\/jats:italic> gene datasets and carried out the functional over\u2010representation analysis using the individual gene expression values. We further, constructed the gene interaction networks of antibiotic resistance genes and virulence genes of <jats:italic>Mtb<\/jats:italic> to understand the impact of the antibiotics at the molecular level and thus to understand the antimicrobial resistance and virulence patterns. Our study elucidates the impact of antibiotics on the <jats:italic>Mtb<\/jats:italic> genes at the molecular level and the positively enriched pathways, operons, and regulons data are helpful in understanding the resistance patterns in <jats:italic>Mtb<\/jats:italic>. The upregulated genes during the exposure of Bedaquiline and Capreomycin can be considered as potent drug targets for the development of new anti\u2010TB drugs.<\/jats:p>","DOI":"10.1002\/jcb.28711","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T06:31:03Z","timestamp":1555396263000},"page":"14499-14509","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Impact of bedaquiline and capreomycin on the gene expression patterns of multidrug\u2010resistant <i>Mycobacterium tuberculosis<\/i> H37Rv strain and understanding the molecular mechanism of antibiotic resistance"],"prefix":"10.1002","volume":"120","author":[{"given":"Sravan Kumar","family":"Miryala","sequence":"first","affiliation":[{"name":"Department of Bio\u2010Sciences, Medical and Biological Computing Laboratory, School of Bio Sciences and Technology Vellore Institute of Technology Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2216-7488","authenticated-orcid":false,"given":"Anand","family":"Anbarasu","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010Sciences, Medical and Biological Computing Laboratory, School of Bio Sciences and Technology Vellore Institute of Technology Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4800-329X","authenticated-orcid":false,"given":"Sudha","family":"Ramaiah","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010Sciences, Medical and Biological Computing Laboratory, School of Bio Sciences and Technology Vellore Institute of Technology Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"e_1_2_9_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052548299"},{"key":"e_1_2_9_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201000071"},{"key":"e_1_2_9_1_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22329"},{"key":"e_1_2_9_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2017.11.028"},{"issue":"2017","key":"e_1_2_9_1_6_1","article-title":"Genomics Exploring the multidrug resistance in Escherichia coli O157: H7 by gene interaction network: a systems biology approach","author":"Miryala SK","year":"2018","journal-title":"Genomics"},{"key":"e_1_2_9_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25422"},{"key":"e_1_2_9_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.08.068"},{"key":"e_1_2_9_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2014.02.009"},{"key":"e_1_2_9_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2006.05.012"},{"key":"e_1_2_9_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4369"},{"key":"e_1_2_9_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22884-8"},{"key":"e_1_2_9_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12192"},{"key":"e_1_2_9_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1017"},{"key":"e_1_2_9_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw937"},{"key":"e_1_2_9_1_16_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2017.00465","article-title":"M. tuberculosis hypothetical proteins and proteins of unknown function: hope for exploring novel resistance mechanisms as well as future target of drug resistance","volume":"8","author":"Sharma D","year":"2017","journal-title":"Front Microbiol"},{"key":"e_1_2_9_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06583.x"},{"key":"e_1_2_9_1_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06328-11"},{"key":"e_1_2_9_1_19_1","doi-asserted-by":"crossref","unstructured":"MalingaLA StoltzA WaltM Van Der.2016. 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