{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:16:32Z","timestamp":1758633392462,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T00:00:00Z","timestamp":1555977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["131900\/2014\u20105"],"award-info":[{"award-number":["131900\/2014\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011647","name":"Universidade Federal de Lavras","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A large number of natural compounds, such as phenolic compounds, have been scientifically evaluated in the search for enzyme inhibitors. The interactions between the phenolic compound <jats:italic>p<\/jats:italic>\u2010coumaric acid and the enzymes present in snake venoms (used as research tools) were evaluated in vitro and in silico. The <jats:italic>p<\/jats:italic>\u2010coumaric acid was able to inhibit 31% of the phospholipase activity induced by <jats:italic>Bothrops alternatus<\/jats:italic> venom, 27% of the hemolytic activity induced by <jats:italic>B. moojeni<\/jats:italic>, 62.5% of the thrombolytic activity induced by <jats:italic>B. jararacussu<\/jats:italic>, and approximately 27% of the activity thrombosis induced by <jats:italic>Crotalus durissus terrificus<\/jats:italic>. Previous incubation of <jats:italic>p<\/jats:italic>\u2010coumaric acid with the venoms of <jats:italic>B. atrox<\/jats:italic> and <jats:italic>B. jararacussu<\/jats:italic> increased the coagulation time by 2.18 and 2.16\u2010fold, respectively. The activity of serine proteases in <jats:italic>B. atrox<\/jats:italic> and <jats:italic>B. jararacussu<\/jats:italic> venoms was reduced by 60% and 66.34%, respectively. Computational chemistry analyses suggests the specific binding of <jats:italic>p<\/jats:italic>\u2010coumaric acid to the active site of proteases through hydrogen and hydrophobic interactions. The phenolic compound evaluated in this work has great potential in therapeutic use to both prevent and treat hemostatic alterations, because the venom proteins inhibited by the <jats:italic>p<\/jats:italic>\u2010coumaric acid have high homology with human proteins that have a fundamental role in several pathologies.<\/jats:p>","DOI":"10.1002\/jcb.28721","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T05:36:32Z","timestamp":1556084192000},"page":"14594-14603","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Molecular interactions between <i>p<\/i>\u2010coumaric acid and snake venom toxins"],"prefix":"10.1002","volume":"120","author":[{"given":"Pedro H. S.","family":"Cesar","sequence":"first","affiliation":[{"name":"Biochemistry Laboratory, Departament of Chemistry Federal University of Lavras Lavras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus Vin\u00edcius","family":"Cardoso Trento","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Departament of Chemistry Federal University of Lavras Lavras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais A.","family":"Sales","sequence":"additional","affiliation":[{"name":"Computational Chemistry Laboratory, Departament of Chemistry Federal University of Lavras Lavras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara R.","family":"Marques","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Departament of Chemistry Federal University of Lavras Lavras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana A.","family":"Braga","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Departament of Chemistry Federal University of Lavras Lavras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teodorico C.","family":"Ramalho","sequence":"additional","affiliation":[{"name":"Computational Chemistry Laboratory, Departament of Chemistry Federal University of Lavras Lavras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7693-8239","authenticated-orcid":false,"given":"Silvana","family":"Marcussi","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Departament of Chemistry Federal University of Lavras Lavras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,23]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.11.020"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050218"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050221"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2012.08.017"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2008.02.043"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joim.2018.04.005"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/09298673113206660295"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(88)90010-4"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2012.03.018"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2000.1958"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(90)90062-C"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.09.031"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.02.036"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"volume-title":"R: A Language and environment for statistical computing","year":"2012","author":"R. 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