{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:09:20Z","timestamp":1790280560355,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific research project of Gansu Provincial Administration of traditional Chinese Medicine","award":["GZK-2017-26"],"award-info":[{"award-number":["GZK-2017-26"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MicroRNAs (miRNAs) have been reported to play critical roles in the occurrence, progression, and treatment of many cardiovascular diseases. However, the molecular mechanism by which miRNA regulates target gene expression in ischemia\u2010reperfusion (I\/R) injury in acute myocardial infarction (AMI) is not entirely clear. MiR\u2010340\u20105p was reported to be downregulated in acute ischemic stroke. However, it still remains unknown whether miR\u2010340\u20105p is mediated in the pathogenesis process of I\/R injury after AMI. In the present study, male C57BL\/6\u2009J mice and H9C2 cardiomyocytes were used as experimental models. Real\u2010time polymerase chain reaction analysis, Western blot analysis, and the\u00a0terminal deoxynucleotidyl transferase\u2010mediated dUTP nick\u2010end\u2010labeling immunofluorescence staining assay were conducted to examine related indicators in the study. We confirmed that the expression of miR\u2010340\u20105p is downregulated after I\/R in AMI mice and hypoxia\/reperfusion (H\/R)\u2010induced cardiomyocytes. miR\u2010340\u20105p could inhibit apoptosis and oxidative stress in H\/R\u2010induced H9C2 cells via downregulating activator 1 (Act1). The inhibiting action of miR\u2010340\u20105p on H\/R\u2010induced apoptosis and oxidative stress in cardiomyocytes was partially reversed after Act1 overexpression. Moreover, the results showed that the NF\u2010\u03baB pathway may be mediated in the role of miR\u2010340\u20105p on H\/R\u2010induced cardiomyocyte apoptosis and oxidative stress. We demonstrated that upregulation of miR\u2010340\u20105p suppresses apoptosis and oxidative stress induced by H\/R in H9C2 cells by inhibiting Act1. Therapeutic strategies that target miR\u2010340\u20105p, Act1, and the NF\u2010\u03baB pathway could be beneficial for the treatment of I\/R injury after AMI.<\/jats:p>","DOI":"10.1002\/jcb.28723","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T06:25:58Z","timestamp":1555395958000},"page":"14618-14627","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["microRNA\u2010340\u20105p inhibits hypoxia\/reoxygenation\u2010induced apoptosis and oxidative stress in cardiomyocytes by regulating the Act1\/NF\u2010\u03baB pathway"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5510-9503","authenticated-orcid":false,"given":"Dong","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiology Gansu Provincial Hospital of TCM Lanzhou Gansu 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