{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:17:26Z","timestamp":1740104246755,"version":"3.37.3"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T00:00:00Z","timestamp":1557100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471879"],"award-info":[{"award-number":["81471879"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The pluripotency of adipose\u2010derived stem cells (ADSCs) makes them appropriate for tissue repair and wound healing. Owing to the repair properties of autologous platelet\u2013rich gel (APG), which is based on easily accessible blood platelets, its clinical use has been increasingly recognized by physicians. The aim of this study was to investigate the effect of combined treatment with ADSCs and APG on sciatic nerve regeneration after electrical injury. To facilitate the differentiation of ADSCs, glial cell line\u2013derived neurotrophic factor (GDNF) was overexpressed in ADSCs by lentivirus transfection. GDNF\u2010ADSCs were mingled with APG gradient concentrations, and in vitro, cell proliferation and differentiation were examined with 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine staining and immunofluorescence. A rat model was established by exposing the sciatic nerve to an electrical current of 220\u2009V for 3\u2009seconds. Rat hind\u2010limb motor function and sciatic nerve regeneration were subsequently evaluated. Rat ADSCs were characterized by high expression of CD90 and CD105, with scant expression of CD34 and CD45. We found that GDNF protein expression in ADSCs was elevated after Lenti\u2010GDNF transfection. In GDNF\u2010ADSCs\u2010APG cultures, GDNF was increasingly produced while tissue growth factor\u2010\u03b2 was reduced as incubation time was increased. ADSC proliferation was augmented and neuronal nuclei (NeuN) and glial fibrillary acidic protein (GFAP) expression were upregulated in GDNF\u2010ADSCs\u2010APG. In addition, limb motor function and nerve axon growth were improved after GDNF\u2010ADSCs\u2010APG treatment. In conclusion, our study demonstrates the combined effect of ADSCs and APG in peripheral nerve regeneration and may lead to treatments that benefit patients with electrical injuries.<\/jats:p>","DOI":"10.1002\/jcb.28759","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T03:44:41Z","timestamp":1557200681000},"page":"14971-14985","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["GDNF\u2010ADSCs\u2010APG embedding enhances sciatic nerve regeneration after electrical injury in a rat model"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1903-808X","authenticated-orcid":false,"given":"Zhao","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Burns and Cutaneous Surgery, Xijing Hospital Fourth Military Medical University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Burns and Cutaneous Surgery, Xijing Hospital Fourth Military Medical University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,6]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2006.03.027"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2015.1053867"},{"key":"e_1_2_7_1_4_1","first-page":"59","article-title":"Adipose\u2010derived mesenchymal stem cells: biology and potential applications","volume":"129","author":"Minteer D","year":"2013","journal-title":"Adv Biochem Eng 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