{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:23:22Z","timestamp":1787700202003,"version":"build-2784847793"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,4,26]],"date-time":"2019-04-26T00:00:00Z","timestamp":1556236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b1\u2010Mangostin (\u03b1\u2010M) is a commonly used traditional medicine with various biological and pharmacological activities. Our study aimed to explore the effects and mechanism of \u03b1\u2010M in regulating apoptosis of rheumatoid arthritis fibroblast\u2010like synoviocytes (RA\u2010FLS). \u03b1\u2010M of 10 to 100\u2009\u03bcM was used to treat RA\u2010FLS for 24\u2009hours, followed by measuring cell viability and apoptosis. The involvement of reactive oxygen species (ROS) and mitogen\u2010activated protein kinases was detected. Treatment of \u03b1\u2010M promoted apoptosis and reduced viability of RA\u2010FLS in a dose\u2010dependent manner. The mitochondrial membrane potential in RA\u2010FLS was remarkably reduced by \u03b1\u2010M treatment, accompanied by the cytochrome <jats:italic>c<\/jats:italic> accumulation in the cytosol and increased activities of caspase\u20103 and caspase\u20109. Moreover, we found that \u03b1\u2010M treatment promoted ROS production and extracellular signal\u2010regulated kinase 1\/2 (ERK1\/2) phosphorylation. The proapoptotic activity of \u03b1\u2010M in RA\u2010FLS was markedly reversed by the co\u2010induction with the ERK1\/2 inhibitor LY3214996 or ROS scavenger <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine. In conclusion, our studies found that \u03b1\u2010M had remarkable proapoptotic activities in RA\u2010FLS, which is regulated by the induction of ROS accumulation and ERK1\/2 phosphorylation. \u03b1\u2010M may thus have potential therapeutic effects for rheumatoid arthritis.<\/jats:p>","DOI":"10.1002\/jcb.28760","type":"journal-article","created":{"date-parts":[[2019,4,26]],"date-time":"2019-04-26T13:34:21Z","timestamp":1556285661000},"page":"14986-14994","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["\u03b1\u2010Mangostin promotes apoptosis of human rheumatoid arthritis fibroblast\u2010like synoviocytes by reactive oxygen species\u2010dependent activation of ERK1\/2 mitogen\u2010activated protein kinase"],"prefix":"10.1002","volume":"120","author":[{"given":"Xiaoyun","family":"Sheng","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital Lanzhou Gansu PR 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