{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T18:12:30Z","timestamp":1777313550263,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,7,28]],"date-time":"2019-07-28T00:00:00Z","timestamp":1564272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pre\u2010eclampsia (PE), whose pathophysiology and etiology remain undefined, represents a leading consequence of fetal and maternal mortality and morbidity. Oxidative stress (OS) is recognized to involve in this disorder. In this study, we hypothesized that neural cell adhesion molecule 1 (NCAM1) gene silencing would suppress the OS in the pregnancy complicated by PE. Initially, clinical samples were collected for determination of NCAM1 expression in placental tissues and levels of OS products in blood. To assess the regulatory mechanism of NCAM1 knockdown on OS, we used small interfering RNA (siRNA) to silence NCAM1 expression in human umbilical vein endothelial cells (HUVECs). Next, cells were treated with or without hypoxia\/reoxygenation to observe the level changes of OS products and p38 mitogen\u2010activated protein kinase (p38MAPK) pathway\u2010related genes. Finally, an evaluation of HUVEC migration and invasion abilities was conducted by wound\u2010healing and transwell assays. Placenta of pregnancy with PE presented significantly increased NCAM1 expression in comparison to placenta of normal pregnancy. Meanwhile, enhanced OS in blood of pregnant women with PE was observed relative to women with normal pregnancy. siRNA\u2010mediated knockdown of NCAM1 gene could inhibit the p38MAPK signaling pathway, repress OS, and promote cell migration and invasion in HUVECs, indicating that NCAM1 inhibition could reduce the influence of PE. Importantly, blocking the p38MAPK signaling pathway reversed the inhibitory role of NCAM1 gene silencing on PE. Collectively, this study defines potential role of NCAM1 gene silencing as a therapeutic target in PE through inhibiting OS and enhancing HUVEC migration and invasion by disrupting the p38MAPK signaling pathway.<\/jats:p>","DOI":"10.1002\/jcb.28778","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T06:28:41Z","timestamp":1564381721000},"page":"18608-18617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["siRNA\u2010mediated NCAM1 gene silencing suppresses oxidative stress in pre\u2010eclampsia by inhibiting the p38MAPK signaling pathway"],"prefix":"10.1002","volume":"120","author":[{"given":"Xiao\u2010Lin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Obstetrics Zoucheng Hospital District of Affiliated Hospital of Jining Medical University Zoucheng People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2010Xian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Zoucheng Hospital District of Affiliated Hospital of Jining Medical University Zoucheng People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4388-5008","authenticated-orcid":false,"given":"Xiao\u2010Yun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Jining Medical University Jining People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,28]]},"reference":[{"key":"e_1_2_10_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2719"},{"key":"e_1_2_10_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00070-7"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1515\/hmbci-2015-0063","article-title":"Dydrogesterone support following assisted reproductive technique (ART) reduces the risk of pre\u2010eclampsia","volume":"27","author":"Ali AB","year":"2016","journal-title":"Horm Mol Biol Clin Investig"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/140851"},{"key":"e_1_2_10_1_6_1","first-page":"334","article-title":"An overview of recent advances in pathogenesis and diagnosis of preeclampsia","volume":"6","author":"Savaj S","year":"2012","journal-title":"Iran J Kidney Dis"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2008.06.003"},{"key":"e_1_2_10_1_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.i1753"},{"key":"e_1_2_10_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgyn.2016.02.012"},{"key":"e_1_2_10_1_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327114559992"},{"key":"e_1_2_10_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0756.2011.01771.x"},{"key":"e_1_2_10_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-017-0486-0"},{"key":"e_1_2_10_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.04.011"},{"key":"e_1_2_10_1_14_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.35.624"},{"key":"e_1_2_10_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2012.01.008"},{"key":"e_1_2_10_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-017-0079-2"},{"key":"e_1_2_10_1_17_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.061035"},{"key":"e_1_2_10_1_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173711"},{"key":"e_1_2_10_1_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2014.0055"},{"key":"e_1_2_10_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25890"},{"key":"e_1_2_10_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.12.027"},{"key":"e_1_2_10_1_22_1","unstructured":"2010 McGraw Hill New York USA F Cunningham K Leveno S Bloom Williams Obstetrics"},{"key":"e_1_2_10_1_23_1","first-page":"3403","article-title":"Long noncoding RNA UCA1 modulates breast cancer cell growth and apoptosis through decreasing tumor suppressive miR\u2010143","volume":"19","author":"Tuo YL","year":"2015","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_10_1_24_1","doi-asserted-by":"publisher","DOI":"10.5830\/CVJA-2016-047"},{"key":"e_1_2_10_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-010-9392-7"},{"key":"e_1_2_10_1_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.111.00250"},{"key":"e_1_2_10_1_27_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1482.131406"},{"key":"e_1_2_10_1_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.239210"},{"key":"e_1_2_10_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2017.03.007"},{"key":"e_1_2_10_1_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/YPG.0b013e328012d850"},{"key":"e_1_2_10_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2012.04.172"},{"key":"e_1_2_10_1_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00372"},{"key":"e_1_2_10_1_33_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/187208"},{"key":"e_1_2_10_1_34_1","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2011.601367"},{"key":"e_1_2_10_1_35_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152510791170951"},{"key":"e_1_2_10_1_36_1","doi-asserted-by":"publisher","DOI":"10.3109\/10641963.2015.1081226"},{"key":"e_1_2_10_1_37_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090437"},{"key":"e_1_2_10_1_38_1","first-page":"94","article-title":"Correlation of oxidized low\u2010density lipoprotein and lectin\u2010like oxidized low\u2010density lipoprotein receptor\u20101 with pre\u2010eclampsia","volume":"44","author":"Zhang Y","year":"2009","journal-title":"Zhonghua Fu Chan Ke Za Zhi"},{"key":"e_1_2_10_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2008.11.020"},{"key":"e_1_2_10_1_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1164-z"},{"key":"e_1_2_10_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2017.08.015"},{"key":"e_1_2_10_1_42_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI82658"},{"key":"e_1_2_10_1_43_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b16-00896"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28778","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.28778","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28778","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T12:41:55Z","timestamp":1721565715000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.28778"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,28]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1002\/jcb.28778"],"URL":"https:\/\/doi.org\/10.1002\/jcb.28778","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,28]]},"assertion":[{"value":"2018-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}