{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:13Z","timestamp":1790553613666,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ankylosing spondylitis (AS) refers to a type of arthritis manifested with chronic inflammation of spine joints. microRNAs (MiRNAs) have been identified as new therapeutic targets for inflammatory diseases. In this study, we evaluated the influence of microRNA\u201096 (miR\u201096) on osteoblast differentiation together with bone formation in a murine model of AS. The speculated relationship that miR\u201096 could bind to sclerostin (SOST) was verified by dual luciferase reporter assay. After successful model establishment, the mice with AS and osteoblasts isolated from mice with AS were treated with mimics or inhibitors of miR\u201096, or DKK\u20101 (a Wnt signaling inhibitor). The effects of gain\u2010 or loss\u2010of\u2010function of miR\u201096 on the inflammatory cytokine release (IL\u20106, IL\u201010, and TNF\u2010\u03b1), alkaline phosphatase (ALP) activity, calcium nodule formation, along with the viability of osteoblasts were determined. It was observed that miR\u201096 might target and regulate SOST. Besides, miR\u201096 was expressed at a high level in AS mice while SOST expressed at a low level. TOP\/FOP\u2010Flash luciferase reporter assay confirmed that miR\u201096 activated the Wnt signaling pathway. Moreover, AS mice overexpressing miR\u201096 exhibited increased contents of IL\u20106, IL\u201010 and TNF\u2010\u03b1, ALP activity, calcium nodule numbers, and viability of osteoblasts. In contrast, inhibition of miR\u201096 resulted in suppression of the osteoblast differentiation and bone formation. In conclusion, the study implicates that overexpressing miR\u201096 could improve osteoblast differentiation and bone formation in AS mice via Wnt signaling pathway activation, highlighting a potential new target for AS treatment.<\/jats:p>","DOI":"10.1002\/jcb.28810","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T04:30:28Z","timestamp":1558413028000},"page":"15429-15442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["microRNA\u201096 promotes osteoblast differentiation and bone formation in ankylosing spondylitis mice through activating the Wnt signaling pathway by binding to SOST"],"prefix":"10.1002","volume":"120","author":[{"given":"Shu","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Rheumatology The First Hospital of Jilin University Changchun People's Republic of 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