{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:25:00Z","timestamp":1791469500631,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601926"],"award-info":[{"award-number":["81601926"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b2\u2010amyloid peptide (A\u03b2) deposition derived from sequential cleavage of the amyloid precursor protein (APP) through the amyloidogenic pathway is an important characteristic feature of Alzheimer's disease (AD). During this process, cellular trafficking plays a crucial role. A large Sec7\u2010domain containing ADP\u2010ribosylation factor guanine nucleotide exchange factor (ARF\u2010GEF), Golgi brefeldin A resistance factor 1 (GBF1) has been reported to initiate the ADP\u2010ribosylation factor (Arf) activation cascade at trans\u2010Golgi network, which plays a crucial function at the endoplasmic reticulum\u2010Golgi interface. In this study, we investigated the role of GBF1 in APP transmembrane transport and A\u03b2 formation. Using APP\/PS1 (presenilin 1) overexpressing transgenic mice, we demonstrate that GBF1 has upregulated the expression of APP, indicating a role for GBF1 in APP physiological process. Knocking down of GBF1 using small interfering has significantly increased the intracellular but not the surface expression of APP. In contrast, overexpression of wild\u2010type (WT) and guanine nucleotide exchange factor (GEF) in the activated form but not the GEF deficient mutation induced continuous activation of GBF1, which subsequently increased the surface level of APP. Interestingly, inhibition of GBF1 by c(BFA) also impaired APP trafficking and induced endoplasmic reticulum (ER) stress in SH\u2010SY5Y cells. Our results thus for identified the role of GBF1 in APP trafficking and cleavage, and provide evidence for GBF1 as a possible therapeutic target in AD.<\/jats:p>","DOI":"10.1002\/jcb.28827","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T04:29:18Z","timestamp":1558412958000},"page":"15604-15615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Regulatory role of Golgi brefeldin A resistance factor\u20101 in amyloid precursor protein trafficking, cleavage and A\u03b2 formation"],"prefix":"10.1002","volume":"120","author":[{"given":"Kaifei","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, SMU\u2010KI United Medical Inflammatory Center, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xinhua College of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, SMU\u2010KI United Medical Inflammatory Center, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kutty Selva","family":"Nandakumar","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, SMU\u2010KI United Medical Inflammatory Center, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4828-3268","authenticated-orcid":false,"given":"Xiaoyan","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Pharmacy, Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahe","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, SMU\u2010KI United Medical Inflammatory Center, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuixian","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, SMU\u2010KI United Medical Inflammatory Center, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chonghua","family":"He","sequence":"additional","affiliation":[{"name":"Longwoodgene Biotechology, Inc Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7395-5842","authenticated-orcid":false,"given":"Chun","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, SMU\u2010KI United Medical Inflammatory Center, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,20]]},"reference":[{"key":"e_1_2_7_1_2_1","first-page":"2","article-title":"Revisiting the cholinergic hypothesis in Alzheimer's disease: emerging evidence from translational and clinical research","volume":"6","author":"Hampel H","year":"2019","journal-title":"J Prev Alzheimers Dis"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.05015-11"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1385\/JMN:11:2:141"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.03.017"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00221-011-2870-1"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.02.008"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2004.09.018"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32449-3"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-6-30"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304122200"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2008.11.004"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(96)10027-1"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.20.010403.105307"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2005.02.007"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2004.06.011"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.31108.x"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-12-1042"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3117"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.02.006"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.453621"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.205492"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-014-1602-7"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2007.00634.x"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.30705.x"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.210245"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2389"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.438481"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02-11-0730"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/boc.201700042"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01244-09"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01190-09"},{"key":"e_1_2_7_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01482.x"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.09.045"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.112.300720"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1168979"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.100656"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.06.002"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.3852\/15-309"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.05.014"},{"key":"e_1_2_7_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2015.07.017"},{"key":"e_1_2_7_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dib.2015.08.023"},{"key":"e_1_2_7_1_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08805"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28827","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.28827","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T06:04:07Z","timestamp":1721628247000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.28827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,20]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1002\/jcb.28827"],"URL":"https:\/\/doi.org\/10.1002\/jcb.28827","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,20]]},"assertion":[{"value":"2018-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}