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Spheroids on day 9 with 300\u2009\u00b5m diameters were treated with 20\u2009\u00b5M resveratrol and\/or 1\u2009\u00b5M 5\u2010FU for one volume doubling time (VDT) (42\u2009hours) and then irradiated with 2\u2009Gy gamma radiation (<jats:sup>60<\/jats:sup>Co) in various groups. Then the viability of the cells and clonogenic ability were acquired by blue dye exclusion and colony formation assay, respectively. The population doubling time in the monolayer culture and the VDT of spheroid culture was 22.48\u2009\u20090.23\u2009hours and 42\u2009\u20090.63\u2009hours respectively. None of the drugs and combination of them had any effect on the viability of cells. The combination treatment of 5\u2010FU+Res+\u2009radiation significantly reduced the colony formation ability of spheroid cells in comparison with each treatment alone. Our results indicated that resveratrol can significantly decrease colony number of breast cancer spheroid cells treated with 5\u2010FU in combination with gamma\u2010rays. Thus, resveratrol as a hypoxia\u2010inducible factor\u20101\u2010alpha inhibitor increased the radiosensitization of breast cancer spheroid cells.<\/jats:p>","DOI":"10.1002\/jcb.28836","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T05:56:14Z","timestamp":1557381374000},"page":"15671-15677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effect of resveratrol on the radiosensitivity of 5\u2010FU in human breast cancer MCF\u20107 cells"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1083-1409","authenticated-orcid":false,"given":"Shahin","family":"Aghamiri","sequence":"first","affiliation":[{"name":"Student research committee, Department of medical biotechnology, School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Jafarpour","sequence":"additional","affiliation":[{"name":"Students' Scientific Research Center, Virology Division, Department of Pathobiology, School of Public Health Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farshid","family":"Zandsalimi","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering, Faculty of New Sciences and Technologies University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehran","family":"Aghemiri","sequence":"additional","affiliation":[{"name":"Department of Medical Informatics Tarbiat Modares University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Shoja","sequence":"additional","affiliation":[{"name":"Faculty of Paramedicine Semnan University of Medical Sciences Semnan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,8]]},"reference":[{"key":"e_1_2_9_1_2_1","unstructured":"Stewart B. & Wild C. 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