{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:23:10Z","timestamp":1770963790089,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,12]],"date-time":"2019-05-12T00:00:00Z","timestamp":1557619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study performed the first microarray analysis of long\u2010noncoding RNA (lncRNA) and mRNA expression profiles in human steroid\u2010induced avascular necrosis of the femoral head (SAVNFH). Expression levels of lncRNAs and mRNAs in three human SAVNFH samples and three human femoral head fracture samples (controls) were detected using third\u2010generation lncRNA microarrays (KangChen Biotech, Shanghai, China). The fold change, false discovery rate, and <jats:italic>P<\/jats:italic> value were utilized to filter genes with significant differential expression in the SAVNFH samples compared with the control samples. In total, there were 1179 upregulated and 3214 downregulated lncRNAs (P2. zerofold, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Meanwhile, 1092 upregulated and 565 downregulated mRNAs were found in the SAVNFH samples compared with the control samples. Then, quantitative real\u2010time polymerase chain reaction was used to confirm the previous microarray results using 8 and 20 selected dysregulated lncRNAs and mRNAs, respectively, and the results generally confirmed the microarray findings. Finally, we used Gene Ontology (GO) and pathway analysis to investigate the functions of the altered mRNAs and their associated GO terms and biological pathways. The Immune system process term (GO:0002376) was the most significantly upregulated GO term, and the Regulation of blood coagulation term (GO:0030193) was the most significantly downregulated GO term in the biological process category for the SAVNFH samples. \u201cHematopoietic cell lineage \u2010 <jats:italic>Homo sapiens<\/jats:italic> (human) (Pathway ID: hsa04640)\u201d and \u201cComplement and coagulation cascades \u2010 <jats:italic>Homo sapiens<\/jats:italic> (human) (Pathway ID: hsa04610)\u201d were the most significantly up\u2010 and downregulated pathways in the SAVNFH samples compared with the controls. In conclusion, the differential expression of lncRNAs and mRNAs may be correlated with the pathogenesis of SAVNFH, and these significantly dysregulated lncRNAs and mRNAs may function through networks or participate in several specific biological processes. Further research is needed to understand their exact functions and mechanisms in SAVNFH.<\/jats:p>","DOI":"10.1002\/jcb.28850","type":"journal-article","created":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T06:15:37Z","timestamp":1557728137000},"page":"15800-15813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Microarray analysis of long\u2010noncoding RNAs and mRNA expression profiles in human steroid\u2010induced avascular necrosis of the femoral head"],"prefix":"10.1002","volume":"120","author":[{"given":"Hongbin","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Joint Surgery and Sports Medicine the First Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"The School of Pharmacy Fujian Medical University Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weibin","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Longyan First Hospital Affiliated to Fujian Medical University Longyan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongfeng","family":"Li","sequence":"additional","affiliation":[{"name":"The School of the First Clinical Medical Fujian Medical University Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3542-2380","authenticated-orcid":false,"given":"Xiaolan","family":"Lian","sequence":"additional","affiliation":[{"name":"Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology Fujian Medical University Union Hospital Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"The School of Pharmacy Fujian Medical University Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhua","family":"Lin","sequence":"additional","affiliation":[{"name":"The School of Pharmacy Fujian Medical University Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5479-7196","authenticated-orcid":false,"given":"Peng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery and Sports Medicine the First Affiliated Hospital of Fujian Medical University Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,12]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.5312\/wjo.v6.i8.590"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/os.12302"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020\u20101383(16)30835\u2010X"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2015.03.036"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2008.10.001"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00132\u2010017\u20103408\u20104"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795\u2010007\u20100367\u20106"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2004.10.003"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370214566565"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.03.004"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.85.8.2907"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/08941939.2017.1385663"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-799X-6-62"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.5507\/bp.2013.080"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2013.10.014"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.89"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-014-3173-y"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198\u2010019\u201004835\u20109"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mgg3.519"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.01.053"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3606"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.03.077"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2018.01.003"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.05.030"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bav082"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2016.01.033"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301\u2010472X(02)00806\u20108"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.160564"},{"key":"e_1_2_8_1_30_1","article-title":"LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation","volume":"8","author":"Wang Q","year":"2018","journal-title":"Sci Rep"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28850","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.28850","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28850","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T06:05:18Z","timestamp":1721628318000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.28850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,12]]},"references-count":29,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1002\/jcb.28850"],"URL":"https:\/\/doi.org\/10.1002\/jcb.28850","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,12]]},"assertion":[{"value":"2019-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}