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However, the regulatory mechanisms of circRNA as ceRNA associated with the fusion of palatal shelves in palatogenesis are yet unclear. This study aimed to explore the potential mechanism underlying the role of circRNA as ceRNA in cleft palate (CP). First, we systematically analyzed RNA\u2010seq and miRNA\u2010seq data after high\u2010throughput sequencing for embryonic palatal shelf tissues from a mouse CP model induced by maternal exposure to all\u2010trans retinoic acid on embryonic gestation day 14.5 (E14.5). Thirty\u2010nine circRNAs, 18 miRNAs, and 936 messenger RNAs (mRNAs) were significantly dysregulated (log<jats:sub>2<\/jats:sub> [fold change {FC}]\u2009&gt;\u20091; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Thereafter, we constructed a circRNA\u2010associated ceRNA network. Finally, we determined the circRNA_0954\u2013miRNA\u2010881\u20103p\u2010PRKAR1\u03b1 ceRNA network as a hub involved in palatogenesis. Gene Ontology analysis revealed that ceRNA\u2010related genes were associated with facial morphogenesis and developmental gene silencing. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that ceRNA\u2010related genes are involved in apoptosis (<jats:italic>P\u2009&lt;\u2009<\/jats:italic>0.05, fold enrichment &gt;1). Quantitative reverse transcription polymerase chain reaction was performed to verify the results of ceRNA analysis. We found that the circRNA\u2010miRNA\u2010mRNA ceRNA network is involved in palatogenesis. The present results imply that circRNA_0954\u2010miRNA\u2010881\u20103p\u2010PRKAR1\u03b1 ceRNA network may cause dysfunctional palatal fusion and might facilitate the development of novel epigenetic biomarkers to treat CP in the future.<\/jats:p>","DOI":"10.1002\/jcb.28888","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T03:50:41Z","timestamp":1557460241000},"page":"16062-16074","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification of circular RNA\u2010associated competing endogenous RNA network in the development of cleft palate"],"prefix":"10.1002","volume":"120","author":[{"given":"Xuan","family":"Shu","sequence":"first","affiliation":[{"name":"The Cleft Lip and Palate Center Second Affiliated Hospital of Shantou University Medical College Shantou 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