{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:53:40Z","timestamp":1790801620181,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T00:00:00Z","timestamp":1563926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["93\u201003\u201030 to 23200"],"award-info":[{"award-number":["93\u201003\u201030 to 23200"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Acinetobacter baumannii<\/jats:italic> has emerged as a major cause of nosocomial infections. Various resistance mechanisms of <jats:italic>A. baumannii<\/jats:italic> against antibiotics have transformed it into a successful nosocomial pathogen. Because of the limited number of available antibiotics, we used a medicinal plant with an antibacterial effect. <jats:italic>Zataria multiflora<\/jats:italic> Boiss (ZMB) extract and its components were used for the treatment of pneumonic mice infected with <jats:italic>A. baumannii<\/jats:italic>. The biological effects of this extract and the regulation of the outer membrane protein A (ompA) gene were used in a mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A pneumonic mouse model was prepared using clinical and standard strains (1.5\u2009\u00d7\u200910<jats:sup>8<\/jats:sup>\u2009colony\u2010forming units\/mL) of <jats:italic>A. baumannii<\/jats:italic>. BALB\/c mice groups were treated with a ZMB extract, carvacrol, thymol, and sensitive antibiotics. The lung tissues of the treated mice were cultured for 5 days and each day, bacterial clearance and the ompA gene expression were assessed by quantitative real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the lung tissue culture of pneumonic mice infected with standard or clinical isolate, no colony was detected when treated with the ZMB extract after 2 and 3 days (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), respectively. In the carvacrol\u2010treated group, bacterial clearance was seen at day 4 and day 5 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Bacterial clearance was seen 5 days after treatment with thymol and imipenem and 6 days after ampicillin\/sulbactam treatment. The regulation of ompA gene was significantly decreased in this order: ZMB extract, carvacrol, thymol, imipenem, and ampicillin\/sulbactam.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The ZMB extract had a potent bactericidal effect against <jats:italic>A. baumannii<\/jats:italic> that could downregulate the ompA gene. ZBM extract and carvacrol could be novel therapeutic agents for antibiotic\u2010resistant <jats:italic>A. baumannii.<\/jats:italic><\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.28908","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T07:17:24Z","timestamp":1563952644000},"page":"18640-18649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["In vivo antibacterial activity of <i>Zataria multiflora<\/i> Boiss extract and its components, carvacrol, and thymol, against colistin\u2010resistant <i>Acinetobacter baumannii<\/i> in a pneumonic BALB\/c mouse model"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7930-807X","authenticated-orcid":false,"given":"Niloofar","family":"Hassannejad","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Biology Faculty of Sciences Science and Research Brand of Islamic Azad University  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