{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:30:53Z","timestamp":1786354253170,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T00:00:00Z","timestamp":1557792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T00:00:00Z","timestamp":1557792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003121","name":"Ferdowsi University of Mashhad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003121","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diabetes is a devastating metabolic disease that causes long\u2010term damage to various organs. An important leading complication of diabetes is a degenerative effect on the reproductive system including infertility and gonadal dysfunction. This study aimed to evaluate the effects of experimental type I and II diabetes on the levels of luteinizing hormone (LH), follicle\u2010stimulating hormone (FSH), and testosterone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Male Wistar rats were randomly divided into four separate groups: (1) type I diabetes (T1DM), (2) type II diabetes (T2DM), (3) cetrorelix acetate\u2010treated nondiabetic control group, and (4) normal untreated group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096). T1DM was experimentally induced by a single injection of alloxan (135\u2009mg\/kg) while T2DM was induced by feeding the animals with drinking water enriched with fructose (10%). Cetrorelix acetate (100\u2009mg\/kg, intraperitoneal for 1 week) treatment group was used as a positive control. All rats were killed and blood and testes were collected after 8 weeks of the study. The effects of induced diabetes on the levels of blood glucose and insulin were assessed. The levels of sex hormones and insulin were determined by radioimmunoassay. Histological staining was used to check abnormal patterns of testicular morphology, the diameter of seminiferous tubules, testicular diameter, and germinal layer thickness.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A significant reduction in the testosterone, FSH, and LH levels were observed in T1DM, T2DM, and also in cetrorelix acetate\u2010treated groups. Analysis of testicular histology sections revealed significantly reduced thickness of cell layer in T1DM and cetrorelix acetate\u2010treated groups compared with the T2DM group. In T2DM, the cell numbers, the thickness of cell layer, the diameter of seminiferous tubules, and weight of testicles were slightly increased. In contrast, total tubules of empty seminiferous increased significantly in T1D and cetrorelix treated groups compared with the control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, diabetes can induce hypothalamus\u2010pituitary\u2010gonad axis dysfunction, affects hormonal secretion, and causes histological damage to testes, collectively leading to deleterious effects on male fertility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.28936","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T10:29:48Z","timestamp":1557829788000},"page":"16775-16785","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation of testis hormonal and histopathological alterations in type I and type II diabetic rats"],"prefix":"10.1002","volume":"120","author":[{"given":"Saber","family":"Barsiah","sequence":"first","affiliation":[{"name":"Department of Biology Faculty of Science Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morteza","family":"Behnam\u2010Rassouli","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Science Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahimeh","family":"Shahabipour","sequence":"additional","affiliation":[{"name":"National Cell Bank of Iran Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sareh","family":"Rostami","sequence":"additional","affiliation":[{"name":"Neuroscience Research Center Shahid Beheshi University of Medical Science  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad A.","family":"Sabbaghi","sequence":"additional","affiliation":[{"name":"Cancer Research Program IMIM (Hospital del mar Research Institute)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab","family":"Momeni","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Tavassoli","sequence":"additional","affiliation":[{"name":"Division of Biotechnology Faculty of Veterinary Medicine Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8656-1444","authenticated-orcid":false,"given":"Amirhossein","family":"Sahebkar","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center Pharmaceutical Technology Institute Mashhad University of Medical Sciences  Mashhad Iran"},{"name":"Neurogenic Inflammation Research Center Mashhad University of Medical Sciences  Mashhad Iran"},{"name":"School of Pharmacy Mashhad University of Medical Sciences  Mashahd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,14]]},"reference":[{"key":"e_1_2_9_1_2_1","first-page":"284","article-title":"Diabetes mellitus and prothrombotic activity","volume":"56","author":"Mal\u00fd J","year":"2010","journal-title":"Vnitr n\u00ed l\u00e9kar stv\u00ed"},{"key":"e_1_2_9_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO;2-S"},{"key":"e_1_2_9_1_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1478-7954-8-29"},{"key":"e_1_2_9_1_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1567201815666180228161659"},{"key":"e_1_2_9_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2018.01.068"},{"key":"e_1_2_9_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2009.21"},{"key":"e_1_2_9_1_8_1","first-page":"376","article-title":"Type 1 diabetes and autoimmune polyglandular syndrome: a clinical review","volume":"67","author":"den Driessche A","year":"2009","journal-title":"Neth J Med"},{"key":"e_1_2_9_1_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.1.102"},{"key":"e_1_2_9_1_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.150844"},{"key":"e_1_2_9_1_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808431"},{"key":"e_1_2_9_1_12_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc12-0298"},{"key":"e_1_2_9_1_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-103575"},{"key":"e_1_2_9_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dem077"},{"key":"e_1_2_9_1_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/db12-0539"},{"key":"e_1_2_9_1_16_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1527"},{"key":"e_1_2_9_1_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/157339910791658907"},{"key":"e_1_2_9_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.05.005"},{"key":"e_1_2_9_1_19_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc08-0851"},{"key":"e_1_2_9_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60602-5"},{"key":"e_1_2_9_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-1097-5"},{"key":"e_1_2_9_1_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000314193"},{"key":"e_1_2_9_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2009.212"},{"key":"e_1_2_9_1_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.11.8474"},{"key":"e_1_2_9_1_25_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.EJ16-0430"},{"key":"e_1_2_9_1_26_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2011.90527"},{"key":"e_1_2_9_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12575-009-9000-5"},{"key":"e_1_2_9_1_28_1","unstructured":"1979 Academic Press New York U Goebelsmann G Bernstein J Gale Serum gonadotropin testosterone estradiol and estrone levels prior to and following bilateral vasectomy 165"},{"key":"e_1_2_9_1_29_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-36-5-867"},{"key":"e_1_2_9_1_30_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.108.005694"},{"key":"e_1_2_9_1_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2010.01831.x"},{"key":"e_1_2_9_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.04.046"},{"key":"e_1_2_9_1_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/153573"},{"key":"e_1_2_9_1_34_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.295.11.1288"},{"key":"e_1_2_9_1_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-09597-4_14"},{"key":"e_1_2_9_1_36_1","doi-asserted-by":"publisher","DOI":"10.1159\/000200077"},{"key":"e_1_2_9_1_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1993.tb01659.x"},{"key":"e_1_2_9_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02845.x"},{"key":"e_1_2_9_1_39_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1310049"},{"key":"e_1_2_9_1_40_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-978800"},{"key":"e_1_2_9_1_41_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD09081"},{"key":"e_1_2_9_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2009.02.009"},{"key":"e_1_2_9_1_43_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.2.338"},{"key":"e_1_2_9_1_44_1","first-page":"5063","article-title":"GnRH antagonists in the treatment of advanced prostate cancer","volume":"17","author":"Pommerville PJ","year":"2010","journal-title":"Can J Urol"},{"key":"e_1_2_9_1_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-09597-4_1"},{"key":"e_1_2_9_1_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-09597-4_5"},{"key":"e_1_2_9_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00173-9"},{"key":"e_1_2_9_1_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1368-504X.2004.00389.x"},{"key":"e_1_2_9_1_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2007.00815.x"},{"key":"e_1_2_9_1_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03343739"},{"key":"e_1_2_9_1_51_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.296.2.169-a"},{"key":"e_1_2_9_1_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02845.x"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.28936","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28936","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.28936","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28936","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T08:48:18Z","timestamp":1786351698000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.28936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,14]]},"references-count":51,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/jcb.28936"],"URL":"https:\/\/doi.org\/10.1002\/jcb.28936","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,14]]},"assertion":[{"value":"2018-09-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}