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Many antitumor agents have microbial origins. 5\u2032\u2010\n                    <jats:italic>epi<\/jats:italic>\n                    \u2010SPA\u20106952A is a new 24\u2010membered macrolide isolated from the cultured broth of\n                    <jats:italic>Streptomyces diastatochromogenes<\/jats:italic>\n                    . Therefore, we studied the activity and molecular mechanism of 5\u2032\u2010\n                    <jats:italic>epi<\/jats:italic>\n                    \u2010SPA\u20106952A in human cervical carcinoma HeLa cell. The results showed that 5\u2032\u2010\n                    <jats:italic>epi<\/jats:italic>\n                    \u2010SPA\u20106952A significantly inhibited cell proliferation and migration. In addition, 5\u2032\u2010\n                    <jats:italic>epi<\/jats:italic>\n                    \u2010SPA\u20106952A obviously increased the production of intracellular reactive oxygen species and DNA damage in HeLa cells. Moreover, nuclear shrinkage of cells, decrease in mitochondrial membrane potential, and upregulation of Bax\/Bcl\u20102 ratio resulted in the release of cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    , and activation of caspase\u20109\/3 was observed in HeLa cells treated with 5\u2032\u2010\n                    <jats:italic>epi<\/jats:italic>\n                    \u2010SPA\u20106952A, which means it enhanced the intrinsic mitochondrial apoptosis. Besides, DNA\u2010damage associated proteins poly (ADP\u2010ribose) polymerase (PARP) and p53 were also studied, and the expressions of cleaved\u2010PARP and p53 were drastically increased in HeLa cells treated with 5\u2032\u2010\n                    <jats:italic>epi<\/jats:italic>\n                    \u2010SPA\u20106952A. Furthermore, we confirmed that 5\u2032\u2010\n                    <jats:italic>epi\u2010<\/jats:italic>\n                    SPA\u20106952A affected the survival of HeLa cells by blocking cell cycle progression in the G1 phase. Taken together, the results shows that 5\u2032\u2010\n                    <jats:italic>epi<\/jats:italic>\n                    \u2010SPA\u20106952A significantly inhibited HeLa cells proliferation via intrinsic mitochondrial apoptosis, cell cycle arrest, and blocking cell migration.\n                  <\/jats:p>","DOI":"10.1002\/jcb.28939","type":"journal-article","created":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T04:15:15Z","timestamp":1558066515000},"page":"16811-16823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A natural product enhances apoptosis via mitochondria\/caspase\u2010mediated pathway in HeLa cells"],"prefix":"10.1002","volume":"120","author":[{"given":"Yangyang","family":"Fan","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Chemical Biology School of Pharmacy East China University of Science and Technology  Shanghai 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