{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:30:42Z","timestamp":1786354242041,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:00:00Z","timestamp":1559174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:00:00Z","timestamp":1559174400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Anal abscess is an important complication of anal fissure (AF), whereas interleukin\u20106R (IL\u20106R) has been implicated in the development of abscess. In this study, we aimed to explore the possible molecular mechanisms underlying the regulatory effects of miRNAs on IL\u20106R and other inflammatory factors related to the induction of anal abscess in AF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bioinformatics analysis, luciferase assay, real\u2010time polymerase chain reaction, and Western blot analysis were performed to identify the possible regulatory relationships between IL\u20106R and miR\u2010124\/miR\u2010125a by comparing the differentiated expression of miR\u2010125a, miR\u2010124, tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), interferon\u2010\u03b3 (IFN\u2010\u03b3), and IL\u20104 among different groups of AF patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IL\u20106R messenger RNA (mRNA) was identified as a target gene of miR\u2010124 because the luciferase activity in cells cotransfected with wild\u2010type IL\u20106R and miR\u2010124 mimics was significantly reduced. In addition, the expression of IL\u20106R mRNA and protein was significantly inhibited in the presence of miR\u2010124 or an IL\u20106R inhibitor, confirming the presence of a negative regulatory relationship between miR\u2010124 and IL\u20106R. Moreover, miR\u2010124 and inflammatory factors were differentially expressed in AF patients carrying different genotypes of rs531564 polymorphism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u2010124 and inflammatory factors TNF\u2010\u03b1, IFN\u2010\u03b3, and IL\u20104 may be used as indicators of anal abscess development in AF patients. In addition, miR\u2010124 polymorphism rs531564 is involved with the pathogenesis of anal abscess in AF patients, and the presence of rs531564 may increase the incidence of anal abscess via upregulating the expression of IL\u20106R, TNF\u2010\u03b1, IFN\u2010\u03b3, and IL\u20104.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.28970","type":"journal-article","created":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T00:40:19Z","timestamp":1559263219000},"page":"17098-17107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rs531564 polymorphism in microRNA\u2010214 regulates interleukin\u20106R expression in anal fissure patients to affect the risk of anal abscess formation"],"prefix":"10.1002","volume":"120","author":[{"given":"Jiangang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Second Affiliated Hospital of Shandong First Medical University  Taian Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anorectal Second Affiliated Hospital of Shandong First Medical University  Taian Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiwei","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of General Surgery The Second People's Hospital of Xintai  Xintai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9721-6654","authenticated-orcid":false,"given":"Xiuli","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Dermatology Chinese Medicine Hospital in Qufu  Qufu Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichao","family":"Liu","sequence":"additional","affiliation":[{"name":"Departments of Dermatology Second Affiliated Hospital of Shandong First Medical 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