{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:30:38Z","timestamp":1786354238818,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Medical Health Science and Technology Project of Zhejiang Provincial Health Commission","award":["2015KYA187"],"award-info":[{"award-number":["2015KYA187"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of\u00a0China","award":["Y14H290028"],"award-info":[{"award-number":["Y14H290028"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To explore the role of P2Y6 receptors in the maintenance of neuropathic pain and progression of oxidative stress, we investigated the efficacy of the selective P2Y6 receptors antagonist MRS2578 on the antiallodynic effects and improvement of pathological neuropathic pain\u2010induced oxidative stress, thereby finding a potential therapeutic target in neurological disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>The mechanical allodynia in the ipsilateral spinal dorsal horn (SDH) of rats was observed in rats after chronic constriction injury (CCI). Meanwhile, the messenger RNA (mRNA) levels of biological parameters, including superoxide dismutase (SOD), glutathione (GSH), and heme oxygenase\u20101 (HO\u20101) in the SDH of rats were measured by real\u2010time polymerase chain reaction (RT\u2010PCR). In addition, the mRNA expression and protein levels of P2Y6 were measured by RT\u2010PCR and Western blot assay, respectively. Next, the rats subjected to CCI were intrathecally infused with MRS2578 to block the expression of P2Y6 receptors. The positive expression of P2Y6 receptors was examined by immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In the present study, the results revealed that the P2Y6 expression in the ipsilateral SDH of CCI rats was significantly upregulated. In addition, inhibition of the P2Y6 receptor in SDH increased CCI\u2010induced tactile allodynia. Furthermore, the levels of SOD, GSH, and HO\u20101 which were correlated with oxidative stress produced by CCI were also decreased.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The results demonstrated that inhibition of the P2Y6 receptor can generate antiallodynic effects and improved the pathological neuropathic pain\u2010induced oxidative stress. Thus, this study provides a potential approach for the therapy of neurological disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.28972","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T07:27:58Z","timestamp":1558337278000},"page":"17123-17130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The role of P2Y6 receptors in the maintenance of neuropathic pain and its improvement of oxidative stress in rats"],"prefix":"10.1002","volume":"120","author":[{"given":"Zhen","family":"Wang","sequence":"first","affiliation":[{"name":"Anesthesiology and Pain Department Hangzhou Red Cross Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WenSheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Anesthesiology and Pain Department Hangzhou Red Cross Hospital  Hangzhou 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