{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:30:19Z","timestamp":1786354219777,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T00:00:00Z","timestamp":1558483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T00:00:00Z","timestamp":1558483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702092"],"award-info":[{"award-number":["81702092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700265"],"award-info":[{"award-number":["81700265"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470401"],"award-info":[{"award-number":["81470401"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cardiac c\u2010kit positive cells are cardiac\u2010derived cells that exist within the heart and have a great many protective effects. The senescence of cardiac c\u2010kit positive cells probably leads to cell dysfunction. Bradykinin plays a key role in cell protection. However, whether bradykinin prevents cardiac c\u2010kit positive cells from high\u2010glucose\u2010induced senescence is unknown. Here, we found that glucose treatment causes the premature senescence of cardiac c\u2010kit positive cells. Bradykinin B2 receptor (B2R) expression was declined by glucose\u2010induced senescence. Bradykinin treatment inhibited senescence and reduced intracellular oxygen radicals according to senescence\u2010associated \u03b2\u2010galactosidase staining and 2\u2032,7\u2032\u2010dichlorodihydrofluorescein diacetate staining. Moreover, the mitochondrial membrane potential was damaged, as measured by JC\u20101 staining. The mitochondrial membrane potential was preserved under bradykinin treatment. The concentration of superoxide was decreased, and the concentration of intracellular adenosine triphosphate was increased after bradykinin treatment. Western blot showed that bradykinin leads to AKT and mammalian target of rapamycin (mTOR) phosphorylation and decreased levels of P53 and P16 when compared with glucose treatment alone. Antagonists of B2R, phosphoinositide 3\u2010kinase (PI3K), mTOR, and B2R small interfering RNA prevented the protective effect of bradykinin. P53 antagonist also inhibited the glucose\u2010induced senescence of cardiac c\u2010kit positive cells. In conclusion, bradykinin prevents the glucose\u2010induced premature senescence of cardiac c\u2010kit positive cells through the B2R\/PI3K\/AKT\/mTOR\/P53 signal pathways.<\/jats:p>","DOI":"10.1002\/jcb.29039","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T10:49:12Z","timestamp":1558608552000},"page":"17731-17743","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Bradykinin protects cardiac c\u2010kit positive cells from high\u2010glucose\u2010induced senescence through B2 receptor signaling pathway"],"prefix":"10.1002","volume":"120","author":[{"given":"Cong","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Cardiology Yi Ji Shan hospital affiliated to Wan Nan Medical College  Wuhu AnHui China"},{"name":"Department of Cardiology Zhong Da hospital affiliated to Southeast University  Nanjing JiangSu China"},{"name":"Key Laboratory of Non\u2010coding RNA 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