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TGF\u03b21 is not only abnormally increased during fibrosis but also involved in ECM induction and accumulation. Based on the bioinformative analyses, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and focal adhesion kinase (FAK) signaling pathway might be involved in TGF\u03b21 functions on renal fibrosis development. In the present study, fibrosis was induced in HK\u20102 cells using TGF\u03b21 and PTEN expression was significantly suppressed by 24 or 48 hours TGF\u03b21 treatment. PTEN overexpression in HK\u20102 cells improved TGF\u03b21\u2010induced fibrosis within \u03b1\u2010SMA and E\u2010cadherin. According to the KEGG signaling pathway annotation analyses on microarray profiles (GSE23338 and GSE20247) and immunoblotting validation, FAK signaling might be involved in PTEN functions in TGF\u03b21\u2010induced fibrosis. PTEN overexpression significantly inhibited TGF\u03b21\u2010 or unilateral ureteral obstruction (UUO)\u2010induced FAK signaling pathway activation both in vitro and in vivo; more importantly, PTEN silence enhanced TGF\u03b21\u2010 or UUO\u2010induced fibrosis, while FAK inhibitor PF567721 significantly reversed the effects of PTEN silence, indicating that PTEN exerted its effects on TGF\u03b21\u2010 and UUO\u2010induced fibrotic development in vitro and in vivo via inhibiting FAK signaling pathway. In summary, these findings indicate that PTEN could improve cellular fibrotic changes and renal fibrosis via inhibiting FAK\/AKT signaling pathway. Restoring PTEN expression to target FAK\/AKT signaling pathway might be a potent strategy for renal fibrosis treatment.<\/jats:p>","DOI":"10.1002\/jcb.29057","type":"journal-article","created":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:17:03Z","timestamp":1559175423000},"page":"17887-17897","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["PTEN improve renal fibrosis in vitro and in vivo through inhibiting FAK\/AKT signaling pathway"],"prefix":"10.1002","volume":"120","author":[{"given":"Yongchao","family":"Du","sequence":"first","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peihua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan PR 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