{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:08:13Z","timestamp":1791461293190,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,7,11]],"date-time":"2019-07-11T00:00:00Z","timestamp":1562803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Retinoblastoma (RB) is the most common intraocular malignancy in infants and children. S\u2010phase kinase\u2010associated protein 2 (SKP2) has been unmasked as an oncogene in a great many of carcinomas. The biologic function and the detailed molecular mechanism of SKP2 in RB need to be better understood. In this study, real\u2010time quantitative polymerase chain reaction and Western blot showed the ectopic expression of SKP2 in RB tissues and cell lines. Loss of function assays showed the attenuated cell proliferation in RB as a result of SKP2 knockdown. In addition, bioinformatics analysis predicted the interaction between SKP2 and miR\u2010422a. Luciferase reporter assay and Pearson's correlation analysis validated the negative correlation between miR\u2010422a and SKP2. MiR\u2010422a overexpression led to a decline of SKP2 expression and cell growth in RB. The binding capacity between miR\u2010422a and circ_ODC1 was also predicted by bioinformatics analysis. Pearson's correlation analysis and luciferase reporter assay confirmed that circ_ODC1 is negatively correlated with miR\u2010422a. Silencing circ_ODC1 resulted in a rise in miR\u2010422a expression and RB cell growth. Moreover, reduced cell growth was restored by SKP2 overexpression. In a word, SKP2, induced by circ_ODC1 and miR\u2010422a, promotes RB proliferation. Our new findings in this research might expedite the discovery of novel prognostic markers and therapeutic targets of RB.<\/jats:p>","DOI":"10.1002\/jcb.29177","type":"journal-article","created":{"date-parts":[[2019,7,12]],"date-time":"2019-07-12T06:20:29Z","timestamp":1562912429000},"page":"322-331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["SKP2, positively regulated by circ_ODC1\/miR\u2010422a axis, promotes the proliferation of retinoblastoma"],"prefix":"10.1002","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0485-9029","authenticated-orcid":false,"given":"Shanshan","family":"Du","sequence":"first","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology 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