{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:25:41Z","timestamp":1790717141993,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,7,11]],"date-time":"2019-07-11T00:00:00Z","timestamp":1562803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ischemic heart disease (IHD) is the most occurring cardiovascular\u2010associated disease, which is a primary leading cause of cardiac disability and death worldwide. Myocardial ischemia\/reperfusion injury (MI\/RI) has been linked to IHD\u2010induced cardiomyocytes apoptosis and tissue damage. The clinical studies have indicated that pathophysiologic mechanisms of MI\/RI are associated with reactive oxygen species generation, calcium overload, energy metabolism disorder, neutrophil infiltration, and others. However, the genetic mechanism of MI\/RI remains unclear. In this study, we successfully established the reproducing abnormal heart observed in rat, of IHD\u2010induced MI\/RI post operation. By using these rats, we illustrated that expression of miR\u2010181b\u20105p was increased not only in both hypoxia\/reoxygenation\u2010cultured H9C2 but also heart of myocardial ischemia\/reperfusion (MI\/R) rat. Suppression of the miR\u2010181b\u20105p cardiomyocytes apoptosis and rescued myocardial infarction. Additionally, our data indicated that miR\u2010181b\u20105p negatively regulates the expression of AKT3 and PIK3R3 through directly binding with its 3\u2032\u2010untranslated region. More importantly, suppression of miR\u2010181b\u20105p protects the cardiomyocytes apoptosis and tissue damage from MI\/R via regulation of PIK3R3 and AKT3. Hence, our study indicates that miR\u2010181b\u20105p is essential for MI\/RI via regulation of PI3K\/Akt signaling pathway and could be a potential therapeutic target in IHD.<\/jats:p>","DOI":"10.1002\/jcb.29271","type":"journal-article","created":{"date-parts":[[2019,7,12]],"date-time":"2019-07-12T06:15:35Z","timestamp":1562912135000},"page":"19647-19659","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Inhibition of miR\u2010181b\u20105p protects cardiomyocytes against ischemia\/reperfusion injury by targeting AKT3 and PI3KR3"],"prefix":"10.1002","volume":"120","author":[{"given":"Limei","family":"Yuan","sequence":"first","affiliation":[{"name":"College of Acupuncture and Message Henan University of Traditional Chinese Medicine  Zhengzhou Henan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8369-8261","authenticated-orcid":false,"given":"Lihua","family":"Fan","sequence":"additional","affiliation":[{"name":"Departement of Cardiovascular Third Affiliated Hospital of Henan University of Traditional Chinese Medicine  Zhengzhou Henan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghai","family":"Li","sequence":"additional","affiliation":[{"name":"Departement of Cardiovascular Third Affiliated Hospital of Henan University of Traditional Chinese Medicine  Zhengzhou Henan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Cui","sequence":"additional","affiliation":[{"name":"Departement of Cardiovascular Third Affiliated Hospital of Henan University of Traditional Chinese Medicine  Zhengzhou Henan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuechen","family":"Wang","sequence":"additional","affiliation":[{"name":"Departement of Cardiovascular Third Affiliated Hospital of Henan University of Traditional Chinese Medicine  Zhengzhou Henan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Departement of Cardiovascular Third Affiliated Hospital of Henan University of Traditional Chinese Medicine  Zhengzhou Henan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,11]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2014.26"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61682-2"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31012-1"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1089253211436350"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc4801.100038"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc5001.100039"},{"key":"e_1_2_8_1_8_1","first-page":"68","article-title":"Myocardial necrosis induced by temporary occlusion of a coronary artery in the dog","volume":"70","author":"Jennings RB","year":"1960","journal-title":"Arch Pathol"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.224915"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq256"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2008.12.017"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.me.42.020191.001301"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00809-6"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2013.157"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2011.09.012"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23255"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.964684"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485351"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/532915"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2011.05.004"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81883-8"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.11.014"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-015-9111-8"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-0303"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-523-1_7"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986712800493048"},{"issue":"18","key":"e_1_2_8_1_30_1","first-page":"6109","article-title":"Down\u2010regulation of miR\u2010192 protects against rat ischemia\u2010reperfusion injury after myocardial infarction","volume":"22","author":"Dong J","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27663"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27191"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2017.02.004"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2012.02.002"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvt142"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00484.2012"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.29271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.29271","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.29271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T15:12:35Z","timestamp":1692976355000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.29271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,11]]},"references-count":35,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/jcb.29271"],"URL":"https:\/\/doi.org\/10.1002\/jcb.29271","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,11]]},"assertion":[{"value":"2018-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}