{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:07:08Z","timestamp":1787987228725,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T00:00:00Z","timestamp":1564963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["182300410387"],"award-info":[{"award-number":["182300410387"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have found that increased expression of Nav1.9 and protein kinase C (PKC) contributes to pain hypersensitivity in a couple of inflammatory pain models. Here we want to observe if PKC can regulate the expression of Nav1.9 in dorsal root ganglion (DRG) in rheumatoid arthritis (RA) pain model. A chronic knee joint inflammation model was produced by intra\u2010articular injection of the complete Freund's adjuvant (CFA) in rats. Nociceptive behaviors including mechanical, cold, and heat hyperalgesia were examined. The expression of Nav1.9 and PKC\u03b1 in DRG was detected by a quantitative polymerase chain reaction, Western blot, and immunofluorescence. The in vitro and in vivo effects of a PKC activator (phorbol 12\u2010myristate 13\u2010acetate [PMA]) and a PKC inhibitor (GF\u2010109203X) on the expression of Nav1.9 were examined. Moreover, the effects of PKC modulators on nociceptive behaviors were studied. Increased mechanical, heat, and cold sensitivity was observed 3 to 14 days after CFA injection. Parallel increases in messenger RNA and protein expression of Nav1.9 and PKC\u03b1 were found. Immunofluorescence experiments found that Nav1.9 was preferentially colocalized with IB4+DRG neurons in RA rats. In cultured DRG neurons, PMA increased Nav1.9 expression while GF\u2010109203X prevented the effect of PMA. PMA increased Nav1.9 expression in na\u00efve rats while GF\u2010109203X decreased Nav1.9 expression in RA rats. In na\u00efve rats, PMA caused mechanical and cold hyperalgesia. On the other hand, GF\u2010109203X attenuated mechanical and cold hyperalgesia in RA\u2010pain model. Nav1.9 might be upregulated by PKC\u03b1 in DRG, which contributes to pain hypersensitivity in CFA\u2010induced chronic knee joint inflammation model of RA pain.<\/jats:p>","DOI":"10.1002\/jcb.29322","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T04:14:21Z","timestamp":1565064861000},"page":"768-778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Protein kinase C\u2010\u03b1 upregulates sodium channel Nav1.9 in nociceptive dorsal root ganglion neurons in an inflammatory arthritis pain model of rat"],"prefix":"10.1002","volume":"121","author":[{"given":"Qian","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The Second Affiliated Hospital of Zhengzhou University  Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinping","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medicine Zhengzhou University  Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medicine Zhengzhou University  Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuhua","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medicine Zhengzhou University  Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medicine Zhengzhou University  Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songxue","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medicine Zhengzhou University  Henan 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