{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T10:47:40Z","timestamp":1785840460194,"version":"3.56.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T00:00:00Z","timestamp":1567468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T00:00:00Z","timestamp":1567468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    MicroRNAs (miRs) are short noncoding RNAs that play key regulatory roles in osteoblast differentiation. In this study, the specific regulatory roles of miR\u2010218\u20105p on postmenopausal osteoporosis (PMOP) were investigated. The mouse model of PMOP was established by bilateral ovariectomy, and the injection of miR\u2010218\u20105p mimics significantly relieved PMOP degree. Then, bone marrow mesenchymal stem cells (BMMSCs) isolated from PMOP mice were induced into osteoblasts. When compared with normal BMMSCs\n                    <jats:bold>,<\/jats:bold>\n                    PMOP BMMSCs exhibited significantly lower alkaline phosphatase (ALP) activity and less mineralized nodules, as well as downregulated miR\u2010218\u20105p, Runx2, Osterix, COL1A1, and OCN after induction (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u2009.05). The transfection of miR\u2010218\u20105p mimics, and inhibitor significantly promoted, inhibited the osteoblast differentiation of PMOP BMMSCs, respectively. In addition, COL1A1 was a target of miR\u2010218\u20105p. The transfection of miR\u2010218\u20105p mimics into PMOP BMMSCs significantly upregulated COL1A1 at 14th and 21st day post\u2010induction, but not at 7th day. Our findings suggest miR\u2010218\u20105p may relieve PMOP through promoting the osteoblast differentiation of BMMSCs.\n                  <\/jats:p>","DOI":"10.1002\/jcb.29355","type":"journal-article","created":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T03:01:21Z","timestamp":1567479681000},"page":"1216-1226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["MicroRNA\u2010218\u20105p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells"],"prefix":"10.1002","volume":"121","author":[{"given":"Jianqiang","family":"Kou","sequence":"first","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated 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