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Although, the regulatory roles of currently identified miRNAs in various cancer types including breast cancer have been well documented, there are many as yet undiscovered miRNAs. The aim of the current study was to bioinformatically reanalyze a list of 189 potentially new miRNAs introduced in a previously published paper (PMID: 21346806) and experimentally explore the existence and function of a candidate one: hsa\u2010miR\u2010B43 in breast cancer cells. The sequences of 189 potential miRNAs were re\u2010checked in the miRbase database. Genomic location and conservation of them were assessed with the University of California Santa Cruz (UCSC) genome browser. SSC profiler, RNAfold, miRNAFold, MiPred, and FOMmiR bioinformatics tools were furthermore utilized to explore potential hairpin structures and differentiate real miRNA precursors from pseudo ones. hsa\u2010miR\u2010B43 was finally selected as one of the best candidates for laboratory verification. The expression and function of hsa\u2010miR\u2010B43 were examined by real\u2010time polymerase chain reaction, 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide, and wound\u2010healing assays. DIANA\u2010microT, RNAhybrid and Enrichr tools were used to predict the miRNA target genes and for further enrichment analysis. We could detect the exogenous and endogenous expression of hsa\u2010miR\u2010B43, as a real novel miRNA, in cancer cell lines. Gene Ontology enrichment, pathway analysis and wound\u2010healing assay results furthermore confirmed that a metastasis\u2010related function may be assigned to hsa\u2010miR\u2010B43. Our results introduced hsa\u2010miR\u2010B43, as a novel functional miRNA, which might play a role in the metastatic process. Further studies will be necessary to completely survey the existence and function of hsa\u2010miR\u2010B43 in other cancer types.<\/jats:p>","DOI":"10.1002\/jcb.29367","type":"journal-article","created":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T06:35:06Z","timestamp":1567751706000},"page":"1307-1316","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Bioinformatics prediction and experimental validation of a novel microRNA: hsa\u2010miR\u2010B43 within human\n                    <i>CDH4<\/i>\n                    gene with a potential metastasis\u2010related function in breast cancer"],"prefix":"10.1002","volume":"121","author":[{"given":"Azadeh","family":"Rahimi","sequence":"first","affiliation":[{"name":"Department of Genetics and Molecular Biology, Faculty of Medicine Isfahan University of Medical Sciences  Isfahan 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Faculty of Biological Sciences Tarbiat Modares University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Khanahmad","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology, Faculty of Medicine Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1784-6040","authenticated-orcid":false,"given":"Parvaneh","family":"Nikpour","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology, Faculty of Medicine Isfahan University of Medical Sciences  Isfahan Iran"},{"name":"Child Growth and Development Research Center Research Institute for Primordial Prevention of Non\u2010communicable Disease  Isfahan 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