{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:35Z","timestamp":1786977935296,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hepatitis C virus (HCV) infection is a major public health problem, having a high prevalence in Egypt. Leukemia and lymphoma have been associated with HCV infection. MicroRNA\u2010155 (miR\u2010155) has been reported to play a regulatory role in cancer, inflammation, and immune response to infection. The expression level of miR\u2010155 in HCV viremic patients is controversial; although high miR\u2010155 levels were demonstrated in HCV genotypes 1,2, and 3, low levels of miR\u2010155 were detected in Egyptian patients with HCV genotype 4. Several studies have investigated the correlation between the levels of miRNA\u2010155 and the replication of HCV, others have evaluated miRNA\u2010155 as a prognostic biomarker in different types of cancer. No studies have investigated the impact of miRNA\u2010155 knockdown on HCV pediatric patients associated with childhood acute lymphoblastic leukemia (ALL). We knocked\u2010out the miR_155a in cultured polymorphonuclear cells (PBMCs) obtained from 60 children with ALL; 30 were associated with HCV\u20104 infection and 30 were HCV negative. The miR_155a, HCV viral load, and cell proliferation werre assessed in treated and untreated cells using TaqMan assay quantitative polymerase chain reaction. We found that miRNA\u2010155 was significantly upregulated by seven folds in the HCV\u20104 associated ALL group; while being linked to high HCV viral load and leukemic burden, miR_155a knock\u2010out can improve the disease outcome. We conclude that miR\u2010155 is a critical miRNA that is considered a therapeutic target in pediatric HCV leukemic patients.<\/jats:p>","DOI":"10.1002\/jcb.29512","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T06:54:09Z","timestamp":1573109649000},"page":"2811-2817","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["In vitro knock\u2010out of miR\u2010155 suppresses leukemic and HCV virus loads in pediatric HCV\u20104\u2013associated acute lymphoid leukemia: A promising target therapy"],"prefix":"10.1002","volume":"121","author":[{"given":"Safaa S.","family":"Hassan","sequence":"first","affiliation":[{"name":"Clinical Pathology Department, National Cancer Institute Cairo University  Cairo 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