{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:13:34Z","timestamp":1767651214434,"version":"3.37.3"},"reference-count":37,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Special Project of Academic New Seedling Cultivation and Innovation Exploration of Guizhou Medical University in 2018","award":["[2018]5779-30"],"award-info":[{"award-number":["[2018]5779-30"]}]},{"name":"Science and Technology Fund Project of Guizhou Health and Family Planning Commission","award":["gzwjkj-2018-1 to 075","gzwjkj-2018-1 to 035"],"award-info":[{"award-number":["gzwjkj-2018-1 to 075","gzwjkj-2018-1 to 035"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatic fibrosis is a chronic inflammatory and reversible repair reaction of the liver under the continuous action of virus or various injuries. In this study, we aimed at identifying the role of miR\u2010326 in the hepatic stellate cell (HSC) activation and liver fibrosis and its potential mechanism. In this study, the liver fibrosis mouse model was developed by injecting CCl<jats:sub>4<\/jats:sub>. Liver tissue morphology was observed and the expression level of \u03b1\u2010smooth muscle actin, collagen1\u03b11 and miR\u2010326 was measured. Target gene identification was performed by loss\u2010of\u2010function and gain\u2010of\u2010function. The effect of miR\u2010326 on the expression level of the cytokines associated with the TLR4\/MyD88\/nuclear factor\u2010\u03baB (NF\u2010\u03baB) pathway was assessed in vitro and in vivo. We show that miR\u2010326 was downregulated in CCl<jats:sub>4<\/jats:sub>\u2010induced fibrotic mice and activated HSCs. The target gene of miR\u2010326 is TLR4. Moreover, miR\u2010326 inhibited the activation of HSCs in vitro through TLR4\/MyD88\/NF\u2010\u03baB signaling. miR\u2010326 attenuated hepatic fibrosis and inflammation of CCl<jats:sub>4<\/jats:sub>\u2010induced mice in vivo. Our results demonstrate for the first time that miR\u2010326 inhibits HSC activation through TLR4\/MyD88\/NF\u2010\u03baB signaling. Furthermore, miR\u2010326 plays critical roles in attenuating liver fibrosis and inflammation, suggesting the therapeutic potential of miRNAs.<\/jats:p>","DOI":"10.1002\/jcb.29520","type":"journal-article","created":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T12:23:53Z","timestamp":1573043033000},"page":"3794-3803","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["MicroRNA\u2010326 attenuates hepatic stellate cell activation and liver fibrosis by inhibiting TLR4 signaling"],"prefix":"10.1002","volume":"121","author":[{"given":"Xin","family":"Liao","sequence":"first","affiliation":[{"name":"Department of Imaging Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhan","sequence":"additional","affiliation":[{"name":"Surgery of Colorectal Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou 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