{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:35Z","timestamp":1786977935359,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T00:00:00Z","timestamp":1573430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>It is well\u2010recognized that glycemic disorders are leading causes of diabetic complications and acute fluctuation of blood glucose and reported more likely being related to oxidative stress, vasculopathy, and other diabetic complications than continuous hyperglycemia in patients with diabetic\u00a0and animal models. To explore the hypothesis that acute glucose fluctuation (GF) aggravates inflammatory lesions and neuron apoptosis in the hippocampus of diabetic rats. Twenty female GK rats were randomly allocated into a glucose fluctuating group (GK\u2010GF) and a continuous hyperglycemia group (GK\u2010CHG) and 10 age\u2010matched female Wistar rats served as controls. GF was induced in the GK\u2010GF group by injection with glucose and insulin at different periods of time per day for 6 weeks. Body weight was determined weekly. At the end of the study, blood hemoglobin A1c (HbA1c) and serum lipids were measured. Serum and hippocampus interleukin\u00a01\u03b2 (IL\u20101\u03b2), IL\u20106, IL\u20108, and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) were measured by enzyme\u2010linked immunosorbent assay and real\u2010time quantitative reverse transcription\u2010polymerase chain reaction (qRT\u2010PCR). Hippocampus Bcl\u20102, Bax, Pten, fas, and myc were quantified by qRT\u2010PCR and Western blot analysis and Mirror Water Maze (MWM) test was performed. We successfully established an animal model with daily GF and a control model with CHG using GK diabetic rats. The GF and CHG rats showed lower weight gain during the 6\u2010week experimental period with no significant difference in the levels of serum lipids such as total triglyceride, total cholesterol, high\u2010density lipoprotein cholesterol, and low\u2010density lipoprotein cholesterol compared with the control rats at the end of the study. Meanwhile, the GF and CHG rats showed higher blood HbA1c levels than that of control rats. MWM trainings tests detected that glucose disorders in GF and CHG rats tend to present longer latencies, more cross times and longer path length compared with those of the control rats, indicating impaired the hippocampus\u2010regulated behavioral function such as spatial orientating and memory. Importantly, it was found that GF promoted the expression of TNF\u2010\u03b1 and IL\u20101\u03b2 in the hippocampus of the GF rats while continuous hyperglycemia in CHG rats had little effect on that. Furthermore, both GF and CHG diabetic rats had abnormal expression of apoptosis\u2010associated genes in the hippocampus compared with control Wistar rats and neurons apoptosis in GF rats appears to be more severe than CHG rats. Overall, this study confirmed that GF is a more critical factor that would promote the neuron apoptosis and induce inflammation in the hippocampus than continuous hyperglycemia in diabetic animals, which shed new light on the importance of monitoring and administration of blood glucose in the prevention and therapy for diabetes.<\/jats:p>","DOI":"10.1002\/jcb.29523","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:50:33Z","timestamp":1573519833000},"page":"1239-1247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Acute glucose fluctuation induces inflammation and neurons apoptosis in hippocampal tissues of diabetic rats"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1515-3176","authenticated-orcid":false,"given":"Hui","family":"Wang","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of 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