{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T02:50:43Z","timestamp":1775962243260,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T00:00:00Z","timestamp":1573689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81202281"],"award-info":[{"award-number":["81202281"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773530"],"award-info":[{"award-number":["81773530"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Associated with reduced hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) production in Hcy metabolic disorders, Plasma Hcy accumulation can bring about vascular dysfunction. Nevertheless, recently proposed therapies for vascular damage by estrogen could contribute to promoting endogenous hydrogen sulfide production. This study explores whether estrogen can come into play in protection in hyperhomocysteinemia and hypertensive patients at a population level, and then analyses the specific mechanism of estrogen protection in homocysteine (Hcy)\u2010treated human umbilical vein endothelial cells (HUVECs) at the foundational level. A case\u2010control study, conducted on 1277 female hypertension and non\u2010hypertensive patients from Hunan Provincial People's Hospital, showed that the Hcy concentration of hypertensive patients emerged higher than that of healthy controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), and that of estrogen was the reverse (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001). Estrogen had a negative correlation with systolic blood pressure and plasma Hcy concentration. HUVECs were treated with estrogen and Hcy in the basic experimental part, and 17\u03b2\u2010estradiol (E2\u03b2) stimulated proliferation and inhibited damage in Hcy\u2010treated umbilical vein endothelial cells. Treatment with Hcy dampens the expression of cystathionine \u03b2\u2010synthase (CBS) and cystathionine \u03b3\u2010lyase (CSE) then cuts down H<jats:sub>2<\/jats:sub>S production in cultured HUVECs, however, E2\u03b2 reverses this process. To sum up, we have demonstrated a significant correlation between estrogen, Hcy concentration and systolic blood pressure reduction, which is bound up with Hcy metabolism and endogenous hydrogen sulfide production. The role of E2\u03b2 was further strengthened by CBS and the CSE inhibitor through overthrowing the change in hydrogen sulfide of Hcy\u2010treated HUVECs.<\/jats:p>","DOI":"10.1002\/jcb.29527","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T12:26:37Z","timestamp":1573734397000},"page":"915-925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Estradiol\u201017\u03b2 inhibits homocysteine mediated damage by promoting H<sub>2<\/sub>S production via upregulating CBS and CSE expression in human umbilical vein endothelial cells"],"prefix":"10.1002","volume":"122","author":[{"given":"Dandan","family":"Zhang","sequence":"first","affiliation":[{"name":"People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University  Changsha China"},{"name":"Mawangdui Hospital University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2803-8564","authenticated-orcid":false,"given":"Xiuqin","family":"Hong","sequence":"additional","affiliation":[{"name":"People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University  Changsha China"},{"name":"People's Hospital of Hunan Province Emergency Medicine Research Institute  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Wang","sequence":"additional","affiliation":[{"name":"People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[{"name":"People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University  Changsha China"},{"name":"Mawangdui Hospital University of South China  Hengyang China"},{"name":"People's Hospital of Hunan Province Emergency Medicine Research Institute  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University  Changsha China"},{"name":"Mawangdui Hospital University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University  Changsha China"},{"name":"Mawangdui Hospital University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaona","family":"Niu","sequence":"additional","affiliation":[{"name":"People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University  Changsha China"},{"name":"Mawangdui Hospital University of South China  Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jash.2015.02.014"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11403-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph14040376"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2017.11.042"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.05.028"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.268re2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2011"},{"key":"e_1_2_7_9_1","first-page":"1","article-title":"Potential role of the 3\u2010mercaptopyruvate sulfurtransferase (3\u2010MST)\u2010hydrogen sulfide (H2S) pathway in cancer cells","author":"Augsburger F","year":"2018","journal-title":"Pharmacol Res"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.11.048"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2253"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908047106"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2425"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162667"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.010868"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160612560"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000131515.03336.f8"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199307223290406"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1505241"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.07.041"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22546"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2017.09.003"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.790121"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000001304"},{"key":"e_1_2_7_25_1","first-page":"635","article-title":"Association of hypertension with serum estrogen level in postmenopausal women","volume":"26","author":"Yeasmin N","year":"2017","journal-title":"Mymensingh Med J"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.143834"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.191259"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2891-14-6"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8725908"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152510790796156"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389557515666150226114031"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00624"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14100"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000001228"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00245.2016"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00598.2010"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3517"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.06.070186.001143"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/152308603768295249"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-017-0006-7"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2017.01.010"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.29527","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.29527","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.29527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T10:09:06Z","timestamp":1716372546000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.29527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,14]]},"references-count":40,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1002\/jcb.29527"],"URL":"https:\/\/doi.org\/10.1002\/jcb.29527","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,14]]},"assertion":[{"value":"2019-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}