{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T17:01:56Z","timestamp":1770138116779,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2019,11,3]],"date-time":"2019-11-03T00:00:00Z","timestamp":1572739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600484"],"award-info":[{"award-number":["81600484"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Liver fibrosis is a critical pathological process in the early stage of many liver diseases, including hepatic cirrhosis and liver cancer. However, the molecular mechanism is not fully revealed. In this study, we investigated the role of F\u2010box protein 31 (FBXO31) in liver fibrosis. We found FBXO31 upregulated in carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) induced liver fibrosis and in activated hepatic stellate cells, induced by transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2). The enforced expression of FBXO31 caused enhanced proliferation and increased expression of \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) and Col\u20101 in HSC\u2010T6 cells. Conversely, suppression of FBXO31 resulted in inhibition of proliferation and decreased accumulation of \u03b1\u2010SMA and Col\u20101 in HSC\u2010T6 cells. In addition, upregulation of FBXO31 in HSC\u2010T6 cells decreased accumulation of Smad7, the negative regulator of the TGF\u2010\u03b2\/smad signaling pathway, and suppression of the FBXO31 increased accumulation of Smad7. Immunofluorescence staining showed FBXO31 colocalized with Smad7 in HSC\u2010T6 cells and in liver tissues of BALB\/c mice treated with CCl<jats:sub>4<\/jats:sub>. Immunoprecipitation demonstrated FBXO31 interacted with Smad7. Moreover, FBXO31 enhanced ubiquitination of Smad7. In conclusion, FBXO31 modulates activation of HSCs and liver fibrogenesis by promoting ubiquitination of Smad7.<\/jats:p>","DOI":"10.1002\/jcb.29528","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T05:52:30Z","timestamp":1572846750000},"page":"3711-3719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["FBXO31 modulates activation of hepatic stellate cells and liver fibrogenesis by promoting ubiquitination of Smad7"],"prefix":"10.1002","volume":"121","author":[{"given":"Huijuan","family":"He","sequence":"first","affiliation":[{"name":"Clinical Research Center Affiliated Hospital of Guangdong Medical University  Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jialiang","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Hepatological Surgery Affiliated Hospital of Guangdong Medical University  Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jialing","family":"Feng","sequence":"additional","affiliation":[{"name":"The Tumor Branch of Zhongshan People's Hospital  Zhongshan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"He","sequence":"additional","affiliation":[{"name":"Clinical Research Center Affiliated Hospital of Guangdong Medical University  Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuling","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Research Center Affiliated Hospital of Guangdong Medical University  Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Hepatological Surgery Affiliated Hospital of Guangdong Medical University  Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aizhong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of\u00a0General Surgery Anqing Hospital Affiliated to Anhui Medical University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9099-7999","authenticated-orcid":false,"given":"Haili","family":"Huang","sequence":"additional","affiliation":[{"name":"Clinical Research Center Affiliated Hospital of Guangdong Medical University  Zhanjiang China"},{"name":"Department of Hepatological Surgery Affiliated Hospital of Guangdong Medical University  Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.02.015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i25.8048"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2853"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/stke.3992007cm1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2297"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)80994-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000201522"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2011.12.013"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0936"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08011"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.559930"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057530"},{"key":"e_1_2_9_14_1","first-page":"155","article-title":"FBXO31 determines poor prognosis in esophageal squamous cell carcinoma","volume":"39","author":"Kogo R","year":"2011","journal-title":"Int J Oncol"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2183"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-17-0432"},{"key":"e_1_2_9_17_1","first-page":"1814","article-title":"FBXO31 promotes cell proliferation, metastasis and invasion in lung cancer","volume":"5","author":"Huang HL","year":"2015","journal-title":"Am J Cancer Res"},{"key":"e_1_2_9_18_1","first-page":"715","article-title":"FBXO31 is down\u2010regulated and may function as a tumor suppressor in hepatocellular carcinoma","volume":"24","author":"Huang HL","year":"2010","journal-title":"Oncol Rep"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S110969"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.11.029"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.02.089"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.296pe73"},{"key":"e_1_2_9_23_1","first-page":"165","article-title":"Effects of TGF\u2010beta s in the liver: cell proliferation and fibrogenesis","volume":"157","author":"Fausto N","year":"1991","journal-title":"Ciba Found Symp"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/39369"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80303-7"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.24.7151"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/35019643"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)00134-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100008200"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201901200"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.29528","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.29528","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.29528","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T19:01:02Z","timestamp":1693940462000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.29528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,3]]},"references-count":29,"journal-issue":{"issue":"8-9","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1002\/jcb.29528"],"URL":"https:\/\/doi.org\/10.1002\/jcb.29528","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,3]]},"assertion":[{"value":"2019-07-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}