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The aim of this study was to explore the potential functional mechanisms of recombinant human NELL\u20101 protein (rhNELL\u20101) during hASCs osteogenic differentiation. rhNELL\u20101 was added to osteogenic medium to activate osteogenic differentiation of hASCs. High\u2010throughput RNA sequencing (RNA\u2010Seq) was performed and validated by real\u2010time quantitative polymerase chain reaction. Gene ontology functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway analysis were performed to detect the functions of differentially expressed miRNAs and genes. Coding\u2010noncoding gene co\u2010expression network and ceRNA networks were constructed to predict the potential regulatory role of miRNAs. A total of 1010 differentially expressed miRNAs and 1762 differentially expressed messenger RNAs (mRNAs) were detected. miRNA\u2010370\u20103p, bone morphogenetic protein 2 (BMP2), and parathyroid hormone like hormone (PTHLH) were differentially expressed during NELL\u20101\u2010induced osteogenesis. Bioinformatic analyses demonstrated that these differentially expressed miRNAs and mRNAs enriched in Rap1 signaling pathway, PI3K\u2010Akt signaling pathway, p53 signaling pathway, Glucagon signaling pathway, and hypoxia\u2010inducible factor\u20101 signaling pathway, which were important pathways related to osteogenic differentiation. In addition, miRNA\u2010370\u20103p and has\u2010miR\u2010485\u20105p were predicted to interact with circ0001543, circ0002405, and ENST00000570267 in ceRNA networks. Based on the gain or loss of functional experiments by transfection, the results showed that miR\u2010370\u20103p was a key regulator in osteogenic differentiation by targeting BMP2 and disturbing the expression of PTHLH, and participated in NELL\u20101\u2010stimulated osteogenesis. The present study provided the primary data and evidence for further exploration on the roles of miRNAs and ceRNAs during NELL\u20101\u2010induced ossification of hASCs.<\/jats:p>","DOI":"10.1002\/jcb.29695","type":"journal-article","created":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T12:57:44Z","timestamp":1581944264000},"page":"4623-4641","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["microRNA expression profiles and the potential competing endogenous RNA networks in NELL\u20101\u2010induced human adipose\u2010derived stem cell osteogenic differentiation"],"prefix":"10.1002","volume":"121","author":[{"given":"Liyuan","family":"Yu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, Department of Orthodontics West China Hospital of Stomatology, Sichuan University  Chengdu Sichuan 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