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Hepatic cancer is associated with a higher incidence of pathological fracture. However, this important regulatory mechanism remains unexplored. Thus, exosome\u2010mediated cell\u2010cell communication between hepatocellular cancer and bone might be key to osteolytic bone destruction. Huh\u20107 exosomes were characterized for size and exosome marker expressions (CD63, Alix). Exosome mediated osteoclast differentiation in the RAW 264.7 cells was monitored from day 1 to 6 and multinucleated osteoclast formation and bone resorption activity were analyzed. The osteoclastogenic factor expressions in the exosomes and osteoclast differentiation markers such as tumor necrosis factor receptor 6 (TRAF6), nuclear factor \u03baB (NF\u2010\u03baB), nuclear factor of activated T\u2010cells, cytoplasmic 1 (NFATc1), and cathepsin K (CTSK) were analyzed using western blot. Exosomes released by liver cancer cells (Huh\u20107) promoted osteoclast differentiation in RAW 264.7 cells. 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