{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:06:49Z","timestamp":1788908809134,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T00:00:00Z","timestamp":1632787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008353","name":"Funda\u00e7\u00e3o de Apoio ao Ensino, Pesquisa e Assist\u00eancia do Hospital das Cl\u00ednicas da Faculdade de Medicina de Ribeir\u00e3o Preto da Universidade de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008353","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adrenocortical carcinoma (ACC) is a rare, but highly aggressive cancer of the adrenal cortex with a generally poor prognosis. Despite being rare, completely resected ACCs present a high risk of recurrence. Musashi\u20102 (<jats:italic>MSI2<\/jats:italic>) has recently been recognized as a potential prognostic biomarker and therapeutic target in many cancers. However, no studies have evaluated the clinical significance of <jats:italic>MSI2<\/jats:italic> expression in ACC. Here, we addressed <jats:italic>MSI2<\/jats:italic> expression and its association with ACC prognosis and clinicopathological parameters. <jats:italic>MSI2<\/jats:italic> expression was analyzed in TCGA, GSE12368, GSE33371, and GSE49278 ACC datasets; and its correlation with other genes and immune cell infiltration were investigated by using the R2: Genomics Analysis and Visualization Platform and TIMER databases, respectively. Enrichment analysis was performed with the DAVID Functional Annotation Tool. Kaplan\u2013Meier curves, log\u2010rank tests, and Cox regression analyses were used to explore the prognostic role of <jats:italic>MSI2<\/jats:italic> in ACC. Our findings demonstrated the potential value of <jats:italic>MSI2<\/jats:italic> overexpression as an independent predictor of poor prognosis in patients with completely resected ACC (hazard ratio 6.715, 95% confidence interval 1.266\u2009\u2013\u200935.620, <jats:italic>p<\/jats:italic>\u2009=.025). In addition, <jats:italic>MSI2<\/jats:italic> overexpression was associated with characteristics of unfavorable prognosis, such as cortisol excess (<jats:italic>p<\/jats:italic>\u2009=\u2009.002), recurrence (<jats:italic>p<\/jats:italic>\u2009=.003), and death (<jats:italic>p<\/jats:italic>\u2009=.015); positively correlated with genes related to steroid biosynthesis (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05); and negatively correlated with immune\u2010related pathways (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Our findings demonstrate that <jats:italic>MSI2<\/jats:italic> has value as a prognostic marker for completely resected ACC and reinforce the investigation of its role as a possible therapeutic target for patients with ACC.<\/jats:p>","DOI":"10.1002\/jcb.30153","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T12:28:50Z","timestamp":1632832130000},"page":"1925-1935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>MSI2<\/i> expression in adrenocortical carcinoma: Association with unfavorable prognosis and correlation with steroid and immune\u2010related pathways"],"prefix":"10.1002","volume":"122","author":[{"given":"Luciana C.","family":"Veronez","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo F.","family":"das Chagas","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina A. P.","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirella","family":"Baroni","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keteryne R.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis F.","family":"Nagano","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kleiton S.","family":"Borges","sequence":"additional","affiliation":[{"name":"Division of Endocrinology Boston Children's Hospital Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosane G. P.","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz G.","family":"Tone","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Scrideli","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-18-0608"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-019-0221-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2013-1029"},{"issue":"2","key":"e_1_2_10_5_1","first-page":"109","article-title":"Advanced adrenocortical carcinoma: what to do when first\u2010line therapy fails?","volume":"127","author":"Megerle F","year":"2019","journal-title":"Exp Clin Endocrinol Diabetes"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200966"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-2728"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2014.0008"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.3136"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136484"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.03915"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8736"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S298438"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7517"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.3987"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062857"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1183-2"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx247"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.04.002"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-12-0095"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz931"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1349-1"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12010247"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2018-01348"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-1064"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12158"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2016.71"},{"issue":"3","key":"e_1_2_10_31_1","first-page":"2599","article-title":"Increased musashi 2 expression indicates a poor prognosis and promotes malignant phenotypes in gastric cancer","volume":"17","author":"Yang Z","year":"2019","journal-title":"Oncol Lett"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.624"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-020-01587-x"},{"issue":"4","key":"e_1_2_10_34_1","first-page":"5425","article-title":"Musashi\u20102 is a prognostic marker for the survival of patients with cervical cancer","volume":"15","author":"Liu Y","year":"2018","journal-title":"Oncol Lett"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12284"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2016_018"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1513616113"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2019-000469"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.03.023"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.07.008"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2009.416"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01332"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2016.3683"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-265868"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903634"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2013.0149"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.002243"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.30153","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.30153","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.30153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T10:52:12Z","timestamp":1693219932000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.30153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,28]]},"references-count":46,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jcb.30153"],"URL":"https:\/\/doi.org\/10.1002\/jcb.30153","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,28]]},"assertion":[{"value":"2021-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}