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Using whole\u2010genome sequencing and molecular characterisation analysis, the current study reveals the presence of carbapenemase genes in 92.86% of studied Indian isolates. These included\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA\u201051<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA\u201023<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA\u201058<\/jats:sub>\n                    , and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM<\/jats:sub>\n                    genes, with over a third expressing dual carbapenemase genes. As per the MLST scheme, IC2\n                    <jats:sup>Oxf<\/jats:sup>\n                    \/CC2\n                    <jats:sup>Pas<\/jats:sup>\n                    was the predominant clone, with 57.14% isolates belonging to this lineage. The presence of these carbapenemase genes resulted in sulbactam (SUL) resistance (MIC: 16\u2013256\u2009\u00b5g\/ml) in all of the studied isolates. The efficacy of durlobactam (DUR), a novel \u03b2\u2010lactamase inhibitor that also inhibits PBP2 was assessed through in silico intermolecular interaction analysis. Several nonsynonymous single nucleotide polymorphisms were identified in PBP2 (G264S, I108V, S259T) and PBP3 (A515V, T526S) sequences. Minimal variations were recorded in the protein backbone dynamics in active\u2010site motifs of wild\u2010type and mutants, which correlated with negligible binding energy fluctuations for the PBP3\u2010SUL (\u22125.85\u2009\u00b1\u20090.04\u2009kcal\/mol) and PBP2\u2010DUR (\u22125.16\u2009\u00b1\u20090.66\u2009kcal\/mol) complexes. Furthermore, higher binding affinities and low inhibition constants were noted in OXA23\u2010DUR (\u22127.36\u2009kcal\/mol; 4.01\u2009\u00b5M), OXA58\u2010DUR (\u22126.44\u2009kcal\/mol; 19.07\u2009\u00b5M), and NDM\u2010DUR (\u22126.82\u2009kcal\/mol; 10.01\u2009\u00b5M) complexes when compared with the conventional drugs avibactam and aztreonam. Stable interaction profiles of DUR with carbapenemases can possibly restore SUL activity against both PBP3\n                    <jats:sub>WT<\/jats:sub>\n                    and PBP3\n                    <jats:sub>MTs<\/jats:sub>\n                    . The study establishes the efficacy of the novel SUL\u2013DUR combination as a successful treatment strategy in combating emerging XDR strains of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/jcb.30156","type":"journal-article","created":{"date-parts":[[2021,10,2]],"date-time":"2021-10-02T08:53:11Z","timestamp":1633164791000},"page":"1946-1957","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Genome sequencing and molecular characterisation of XDR\n                    <i>Acinetobacter baumannii<\/i>\n                    reveal complexities in resistance: Novel combination of sulbactam\u2013durlobactam holds promise for therapeutic 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