{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:52:44Z","timestamp":1791856364102,"version":"4.3.4"},"reference-count":71,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Chandipura vesiculovirus<\/jats:italic> (CHPV) is a rapidly emerging pathogen responsible for causing acute encephalitis. Due to its widespread occurrence in Asian and African countries, this has become a global threat, and there is an urgent need to design an effective and nonallergenic vaccine against this pathogen. The present study aimed to develop a multi\u2010epitope vaccine using an immunoinformatics approach. The conventional method of vaccine design involves large proteins or whole organism which leads to unnecessary antigenic load with increased chances of allergenic reactions. In addition, the process is also very time\u2010consuming and labor\u2010intensive. These limitations can be overcome by peptide\u2010based vaccines comprising short immunogenic peptide fragments that can elicit highly targeted immune responses, avoiding the chances of allergenic reactions, in a relatively shorter time span. The multi\u2010epitope vaccine constructed using CTL, HTL, and IFN\u2010\u03b3 epitopes was able to elicit specific immune responses when exposed to the pathogen, <jats:italic>in silico<\/jats:italic>. Not only that, molecular docking and molecular dynamics simulation studies confirmed a stable interaction of the vaccine with the immune receptors. Several physicochemical analyses of the designed vaccine candidate confirmed it to be highly immunogenic and nonallergic. The computer\u2010aided analysis performed in this study suggests that the designed multi\u2010epitope vaccine can elicit specific immune responses and can be a potential candidate against CHPV.<\/jats:p>","DOI":"10.1002\/jcb.30170","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T08:07:50Z","timestamp":1635926870000},"page":"322-346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Immunoinformatics based designing a multi\u2010epitope vaccine against pathogenic <i>Chandipura vesiculovirus<\/i>"],"prefix":"10.1002","volume":"123","author":[{"given":"Debashrito","family":"Deb","sequence":"first","affiliation":[{"name":"Department of Life Sciences Garden City University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srijita","family":"Basak","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Garden City University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamalika","family":"Kar","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Garden City University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utkarsh","family":"Narsaria","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Garden City University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Castiglione","sequence":"additional","affiliation":[{"name":"Institute for Applied Computing National Research Council of Italy  Via dei Taurini Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhirup","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Garden City University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashutosh","family":"Pandey","sequence":"additional","affiliation":[{"name":"Plant Metabolic Engineering National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3493-1375","authenticated-orcid":false,"given":"Anurag P.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Garden City University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,3]]},"reference":[{"issue":"12","key":"e_1_2_9_2_1","first-page":"1295","article-title":"Chandipura: a new Arbovirus isolated in India from patients with febrile illness","volume":"55","author":"Bhatt P","year":"1967","journal-title":"Indian J Med Res"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16982-1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874357901812010044"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-38.4.480"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118393277"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5916.191929"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030315"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10540-007-9054-z"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-11-2635"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.02.089"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-39299-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2018.1519460"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2019.06.004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2016.20"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196484"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2007.03.010"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-113"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3290"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1748-7188-9-4"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-424"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-8-30"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines3030579"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-4"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq551"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00894-014-2278-5"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt619"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005053"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.14.70"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.softx.2015.06.001"},{"key":"e_1_2_9_32_1","volume-title":"XMGRACE, Version 5.1. 19","author":"Turner P","year":"2005"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki376"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.3.1281"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009862"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/842329"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004756"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.04.009"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.169"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr040426m"},{"key":"e_1_2_9_41_1","volume-title":"Statistical Mechanics, Chap. 21","author":"McQuarrie D","year":"1976"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01864-13"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.06.001"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.04.063"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6124030"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.04.031"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-013-1516-5"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5517-x"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14686"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9513-4"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.92"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines7030088"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1385\/1592598900"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"issue":"6","key":"e_1_2_9_56_1","first-page":"1895","article-title":"Thermostability and aliphatic index of globular proteins","volume":"88","author":"Ikai A","year":"1980","journal-title":"J Biochem"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2020.101413"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines7020034"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.15.7867-7873.2004"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2010.04196.x"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-125"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00316"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.28371"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.05.008"},{"issue":"1","key":"e_1_2_9_65_1","first-page":"13348","article-title":"Contemplating the importance of Toll\u2010like receptors I and II regarding human viral pathogenesis","volume":"8","author":"Saeed U","year":"2015","journal-title":"Jundishapur J Microbiol"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41496-4"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49481-7"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27189-4"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.9b00944"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2009.10507317"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2014.01.018"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.15171\/bi.2019.06"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.30170","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.30170","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.30170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T12:33:54Z","timestamp":1693139634000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.30170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,3]]},"references-count":71,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/jcb.30170"],"URL":"https:\/\/doi.org\/10.1002\/jcb.30170","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,3]]},"assertion":[{"value":"2021-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}