{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:20:30Z","timestamp":1790803230657,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004481","name":"University of Tehran","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004481","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Permanent degeneration and loss of dopaminergic (DA) neurons in substantia nigra is the main cause of Parkinson's disease. Considering the therapeutic application of stem cells in neurodegeneration, we sought to examine the neurogenic differentiation potential of the newly introduced neural crest originated mesenchymal stem cells (MSCs), namely, trabecular meshwork\u2010derived mesenchymal stem cells (TM\u2010MSCs) compared to two other sources of MSCs, adipose tissue\u2010derived stem cells\u00a0(ADSCs) and bone marrow\u2010derived mesenchymal stem cells (BM\u2010MSCs). The three types of cells were therefore cultured in the presence and absence of a neural induction medium followed by the analysis of their differentiation potentials. Our results showed that TM\u2010MSCs exhibited enhanced neural morphologies as well as higher expressions of <jats:italic>MAP2<\/jats:italic> as the general neuron marker and <jats:italic>Nurr\u20101<\/jats:italic> as an early DA marker compared to the adipose tissue\u2010derived mesenchymal stem cells\u00a0(AD\u2010MSCs) and bone marrow\u2010derived stem cells\u00a0(BMSCs). Also, analysis of Nurr\u20101 immunostaining showed more intense Nurr\u20101 stained nuclei in the neurally induced TM\u2010MSCs compared to those in the AD\u2010MSCs, BMSCs, and noninduced control TM\u2010MSCs. To examine if Wnt\/beta\u2010catenin pathway drives TM\u2010MSCs towards a DA fate, we treated them with the Wnt agonist (CHIR, 3\u2009\u03bcM) and the Wnt antagonist (IWP\u20102, 3\u2009\u03bcM). Our results showed that the expressions of <jats:italic>Nurr\u20101<\/jats:italic> and <jats:italic>MAP2<\/jats:italic>, as well as the Wnt\/beta\u2010catenin target genes, <jats:italic>c\u2010Myc<\/jats:italic> and <jats:italic>Cyclin D1<\/jats:italic>, were significantly increased in the CHIR\u2010treated TM\u2010MSCs, but significantly reduced in those treated with IWP\u20102. Altogether, we declare first\u00a0a higher neural potency of TM\u2010MSCs compared to the more commonly used MSCs, BMSCs and ADSCs, and second\u00a0that Wnt\/beta\u2010catenin activation directs the neurally induced TM\u2010MSCs towards a DA fate.<\/jats:p>","DOI":"10.1002\/jcb.30269","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T18:25:10Z","timestamp":1652120710000},"page":"1120-1129","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Wnt\/\u03b2\u2010catenin signaling pathway is involved in early dopaminergic differentiation of trabecular meshwork\u2010derived mesenchymal stem cells"],"prefix":"10.1002","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4451-089X","authenticated-orcid":false,"given":"Faezeh","family":"Sahebdel","sequence":"first","affiliation":[{"name":"Developmental Biology Laboratory, Department of Animal Biology, School of Biology, College of Science University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1678-2863","authenticated-orcid":false,"given":"Azita","family":"Parvaneh Tafreshi","sequence":"additional","affiliation":[{"name":"Developmental Biology Laboratory, Department of Animal Biology, School of Biology, College of Science University of Tehran  Tehran Iran"},{"name":"Department of Medical Biotechnology National Institute of Genetic Engineering and Biotechnology  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0758-4710","authenticated-orcid":false,"given":"Ehsan","family":"Arefian","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Biology, College of Science University of Tehran  Tehran 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