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Variable outcomes have been associated with several factors including cancer pathology, experimental procedure, MSC source tissue, and individual genetic differences. It is also known that MSCs exert their therapeutic effects with various paracrine factors released from these cells. The profiles of the factors released from MSCs are altered by heat shock, hypoxia, oxidative stress, starvation or various agents such as inflammatory cytokines, and their therapeutic potential is affected. In this study, the antitumor potential of conditioned media (CM), which contains paracrine factors, of mild hyperthermia\u2010stimulated mesenchymal stromal cells derived from lymphoid organ tonsil tissue (T\u2010MSC) was investigated in comparison with CM obtained from T\u2010MSCs grew under normal culture conditions. CM was obtained from T\u2010MSCs that were successfully isolated from palatine tonsil tissue and characterized. The cytotoxic effect of CM on the growth of hematological cancer cell lines at different concentrations (1:1 and 1:2) was demonstrated by methylthiazoldiphenyl\u2010tetrazolium bromide analysis. In addition, the apoptotic effect of T\u2010MSC\u2010CM treatment was evaluated on the cancer cells using Annexin\u2010V\/PI detection method by flow cytometry. The pro\/anti\u2010apoptotic and cytokine\u2010related gene expressions were also analyzed by real\u2010time polymerase chain reaction\u00a0post T\u2010MSC\u2010CM treatment. In conclusion, we demonstrated that the factors released from hyperthermia\u2010stimulated T\u2010MSCs induced apoptosis in hematological cancer cell lines in a dose\u2010dependent manner. Importantly, our results at the transcriptional level support that the factors and cytokines released from hyperthermia\u2010stimulated T\u2010MSC may exert antitumoral effects in cancer cells by downregulation of IL\u20106 that promotes\u00a0tumorigenesis. These findings reveal that T\u2010MSC\u2010CM can be a powerful cell\u2010free therapeutical strategy for cancer therapy.<\/jats:p>","DOI":"10.1002\/jcb.30322","type":"journal-article","created":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T18:34:45Z","timestamp":1661625285000},"page":"1966-1979","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Hyperthermia\u2010stimulated tonsil\u2010mesenchymal stromal cells suppress hematological cancer cells through downregulation of IL\u20106"],"prefix":"10.1002","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-838X","authenticated-orcid":false,"given":"Melek","family":"Y\u00fcce","sequence":"first","affiliation":[{"name":"Stem Cell Research &amp; Application Center Ondokuz May\u0131s University, Kurupelit Campus Atakum\/Samsun 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