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SCs possess unique features that meet germ cells' metabolic demands: they produce lactate from glucose, which is delivered as energy substrate to germ cells. SCs store fatty acids (FAs) as triacylglycerols (TAGs) in lipid droplets (LDs) and can oxidize FAs to sustain their own energetic demands. They also produce ketone bodies from FAs. It has been shown that exposure of SCs to metabolic stresses, such as glucose deprivation, triggers specific adaptive responses that sustain cell survival and preserve lactate supply to germ cells. The aim of the present study was to investigate whether there are modifications in rat SCs lipid metabolism, including LD content, FA oxidation, and ketone bodies production, as part of their adaptive response to glucose deprivation. The present study was performed in 20\u2010day\u2010old rat SCs cultures. We determined LD content by Oil Red O staining, FA oxidation by measuring the release of <jats:sup>3<\/jats:sup>H<jats:sub>2<\/jats:sub>O from [<jats:sup>3<\/jats:sup>H] palmitate, TAGs and 3\u2010hydroxybutyrate levels by spectrophotometric methods, and mRNA levels by RT\u2010qPCR. Results show that the absence of glucose in SC culture medium entails: (1) a decrease in LD content and TAGs levels that is accompanied by decreased perilipin 1 mRNA levels, (2) an increase in FA oxidation that is in part mediated by AMP kinase (AMPK) activation and (3) a decrease in 3\u2010hydroxybutyrate production. Additionally, we studied whether sestrins (SESN1, 2 and 3), proteins involved in the cellular response to stress, are regulated in glucose deprivation conditions. We show that there is an increase in SESN2 mRNA levels in deprived conditions. In conclusion, glucose deprivation affects SC lipid metabolism promoting FA mobilization from LDs to be used as energy source.<\/jats:p>","DOI":"10.1002\/jcb.30399","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T12:14:44Z","timestamp":1679487284000},"page":"716-730","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sertoli cell adaptation to glucose deprivation: Potential role of AMPK in the regulation of lipid metabolism"],"prefix":"10.1002","volume":"124","author":[{"given":"Gustavo M.","family":"Rindone","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u201cDr. C\u00e9sar Bergad\u00e1\u201d (CEDIE)\u2013Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)\u2013FEI\u2013Divisi\u00f3n de Endocrinolog\u00eda Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez Buenos Aires 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