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Therefore, it is imperative to elucidate the mechanisms underlying UTUC growth and metastasis. To explore the function of miR\u2010145\u20105p in UTUC, we transfected the BFTC909 cell line with miR\u2010145\u20105p mimics and analyzed the differences in protein levels by performing two\u2010dimensional polyacrylamide gel electrophoresis. Real\u2010time polymerase chain reaction and Western blot analysis were used to analyze 5\u2010aminoimidazole\u20104\u2010carboxamide ribonucleotide formyltransferase\/inositol monophosphate cyclohydrolase (ATIC) messenger RNA and protein levels. A dual\u2010luciferase assay was performed to identify the target of miR\u2010145\u20105p in ATIC. The effects of miR\u2010145\u20105p and ATIC expression by cell transfection on cell proliferation, migration, and invasion were also assessed. miR\u2010145\u20105p downregulated ATIC protein expression. High ATIC expression is associated with tumor stage, metastasis, recurrence, and a poor prognosis in patients with UTUC. Cell function assays revealed that ATIC knockdown inhibited the proliferation, migration, and invasive abilities of UTUC cells. In contrast, miR\u2010145\u20105p affected the proliferation, migration, and invasive abilities of UTUC cells by directly targeting the 3\u2032\u2010untranslated regions of ATIC. Furthermore, we used RNA sequencing and Ingenuity Pathway Analysis to identify possible downstream genes regulated by ATIC and found that miR\u2010145\u20105p regulated the protein levels of fibronectin 1, Slug, cyclin A2, cyclin B1, P57, and interferon\u2010induced transmembrane 1 via ATIC. ATIC may be a valuable predictor of prognosis and a potential therapeutic target for UTUC.<\/jats:p>","DOI":"10.1002\/jcb.30449","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T06:36:43Z","timestamp":1689921403000},"page":"1324-1345","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["MicroRNA\u2010145\u20105p suppresses cell proliferation, migration, and invasion in upper tract urothelial carcinoma by targeting 5\u2010aminoimidazole\u20104\u2010carboxamide ribonucleotide formyltransferase\/IMP cyclohydrolase"],"prefix":"10.1002","volume":"124","author":[{"given":"Hao\u2010Lun","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Urology Kaohsiung Chang Gung Memorial Hospital and Chang Gung University and College of Medicine Kaohsiung Taiwan"},{"name":"Center for Shockwave Medicine and Tissue Engineering Kaohsiung Chang Gung Memorial Hospital and Chang Gung 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