{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T01:34:43Z","timestamp":1778204083989,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871512"],"award-info":[{"award-number":["31871512"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170863"],"award-info":[{"award-number":["32170863"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>5\u2010Methylcytosine (m<jats:sup>5<\/jats:sup>C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre\u2010eclampsia (PE)\u00a0remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP\u2010seq)\u00a0and RNA\u2010sequencing (RNA\u2010seq) to map m<jats:sup>5<\/jats:sup>C peaks and mRNA expression profile in the decidua of human early\u2010onset PE (EPE), late\u2010onset PE (LPE), and normal pregnancy (NP). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes\u00a0pathway analyses elucidated potential roles of the differentially methylated mRNAs (DMGs) and differentially expressed mRNAs in decidualization pathways. Integrative analysis of MeRIP\u2010seq and RNA\u2010seq data pinpointed 50 candidate genes linked to PE, marked by both differentially methylated m<jats:sup>5<\/jats:sup>C peaks and congruent expression changes. To validate these observations, we selected nine genes for verification via quantitative PCR. Our results underscore the precision and reproducibility of our bioinformatics approach. Importantly, we propose that changes in m<jats:sup>5<\/jats:sup>C modification and expression of relevant mRNA might influence the pathogenesis of PE by hampering decidualization. This work shines light on the distinct mRNA m<jats:sup>5<\/jats:sup>C modification patterns and expression profiles in the decidua of PE, implicating pivotal signaling disruptions and decidualization impediments in the onset of PE.<\/jats:p>","DOI":"10.1002\/jcb.30479","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T14:00:17Z","timestamp":1696514417000},"page":"1720-1733","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Altered 5\u2010methylcytosine modification of mRNA is involved in the pathogenesis of pre\u2010eclampsia"],"prefix":"10.1002","volume":"124","author":[{"given":"Jing","family":"Tong","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine, Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwanyu","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9650-4398","authenticated-orcid":false,"given":"Cong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"},{"name":"Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences Shandong Normal University Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-46011-3"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000437382.03963.88"},{"issue":"2","key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"S886","DOI":"10.1016\/j.ajog.2020.09.039","article-title":"Decidualization resistance in the origin of preeclampsia","volume":"226","author":"Garrido\u2010G\u00f3mez T","year":"2020","journal-title":"Am J Obstet Gynecol"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26221"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1706546114"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2014-1045"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)70770-9"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10020102"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-020-02417-6"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0361-y"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.05.003"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-9-r137"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065598"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2018.05.003"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr261"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.55"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02820507"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1274\/jmor.32.95"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19102848"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1547"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03513-4"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-019-0218-x"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.06.033"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a022061"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-7-80"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2001.0746"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.14257"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.30479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T23:56:25Z","timestamp":1700783785000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.30479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,5]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1002\/jcb.30479"],"URL":"https:\/\/doi.org\/10.1002\/jcb.30479","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,5]]},"assertion":[{"value":"2023-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}