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The aim of the study was to evaluate the role of <jats:italic>PER2<\/jats:italic> during experimental skin wound healing. Two groups (control and <jats:italic>Per2<\/jats:italic>\u2010KO), consisting of 14 male mice each, were anesthetized by inhalation, and two 6\u2009mm wounds were created on their dorsal skin using a punch biopsy. A silicone ring was sutured around the wound perimeter to restrict contraction. The wound healing process was clinically measured daily (closure index) until complete wound repair. On Day 6, histomorphometric analysis was performed using the length and thickness of the epithelial migration tongue, in addition to counting vessels underlying the lesion by immunofluorescence assay and maturation of collagen fibers through picrosirius staining. Bromodeoxyuridine\u00a0(BrdU) incorporation and quantification were performed using the subcutaneous injection technique 2\u2009h before euthanasia and through immunohistochemical analysis of the proliferative index. In addition, the qualitative analysis of myofibroblasts and periostin distribution in connective tissue was performed by immunofluorescence. Statistically significant differences were observed in the healing time between the experimental groups (means: 15.5 days for control mice and 13.5 days for <jats:italic>Per2<\/jats:italic>\u2010KO; <jats:italic>p<\/jats:italic>\u2009=\u20090.001). The accelerated healing observed in the <jats:italic>Per2<\/jats:italic>\u2010KO group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) was accompanied by statistical differences in wound diameter and length of the migrating epithelial tongue (<jats:italic>p<\/jats:italic>\u2009=\u20090.01) compared to the control group. Regarding BrdU immunoreactivity, higher expression was observed in the intact epithelium of <jats:italic>Per2<\/jats:italic>\u2010KO animals (<jats:italic>p<\/jats:italic>\u2009=\u20090.01), and this difference compared to control was also present, to a lesser extent, at the wound site (<jats:italic>p<\/jats:italic>\u2009=\u20090.03). Immunofluorescence in the connective tissue underlying the wound showed a higher angiogenic potential in the <jats:italic>Per2<\/jats:italic>\u2010KO group in the intact tissue area and the wound region (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01), where increased expression of myofibroblasts was also observed. Qualitative analysis revealed the distribution of periostin protein and collagen fibers in the connective tissue underlying the wound, with greater organization and maturation during the analyzed period. Our research showed that the absence of the <jats:italic>Per2<\/jats:italic> gene positively impacts the healing time of the skin in vivo.\u00a0This acceleration depends on the increase of epithelial proliferative and angiogenic capacity of cells carrying the <jats:italic>Per2<\/jats:italic> deletion.<\/jats:p>","DOI":"10.1002\/jcb.30513","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T07:50:19Z","timestamp":1705477819000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Clock gene <i>Per2<\/i> modulates epidermal tissue repair in vivo"],"prefix":"10.1002","volume":"125","author":[{"given":"Veronica Quispe","family":"Yujra","sequence":"first","affiliation":[{"name":"Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, School of Dentistry University of Michigan (UM) Ann Arbor Michigan USA"},{"name":"Department of Biosciences Federal University of S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ericka Janine\u00a0Dantas da","family":"Silveira","sequence":"additional","affiliation":[{"name":"Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, School of Dentistry University of Michigan (UM) Ann Arbor Michigan USA"},{"name":"Odontology Sciences Postgraduate Program, Dentistry Department Federal University of Rio Grande do Norte (UFRN) Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-4983","authenticated-orcid":false,"given":"Daniel Araki","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, School of Dentistry University of Michigan (UM) Ann Arbor Michigan USA"},{"name":"Department of Biosciences Federal University of S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo 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