{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:26Z","timestamp":1753869386210,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T00:00:00Z","timestamp":1705276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Excessive bone\u2010resorbing osteoclast activity during bone remodeling is a major feature of bone diseases, such as osteoporosis. Therefore, the inhibition of osteoclast formation and bone resorption can be an effective therapeutic target for various bone diseases. <jats:italic>Gryllus biomaculatus<\/jats:italic> (GB) has recently been approved as an alternative food source because of its high nutritional value and environmental sustainability. Traditionally, GB has been known to have various pharmacological properties, including antipyretic and blood pressure\u2010lowering activity, and it has recently been reported to have various biological activities, including protective effects against inflammation, oxidative stress, insulin resistance, and alcohol\u2010induced liver injury. However, the effect of GB on osteoclast differentiation and bone metabolism has not yet been demonstrated. In this study, we confirmed the inhibitory effect of GB extract (GBE) on the receptor activator of nuclear factor\u2010\u03baB ligand (RANKL)\u2010induced osteoclast formation. To determine the effect of GBE on RANKL\u2010induced osteoclast differentiation and function, we performed TRAP and F\u2010actin staining, as well as a bone\u2010resorbing assay. The intracellular mechanisms of GBE responsible for the regulation of osteoclastogenesis were revealed by Western blot analysis and quantitative real\u2010time polymerase chain reaction. We investigated the relationship between GBE and expression of osteoclast\u2010specific molecules to further elucidate the underlying mechanisms. It was found that GBE significantly suppressed osteoclastogenesis by decreasing the phosphorylation of Akt, p38, JNK, and ERK, as well as Btk\u2010PLC\u03b32 signaling, in pathways involved in early osteoclastogenesis as well as through the subsequent suppression of c\u2010Fos, NFATc1, and osteoclastogenesis\u2010specific marker genes. Additionally, GBE inhibited the formation of F\u2010actin ring\u2010positive osteoclasts and bone resorption activity of mature osteoclasts. Our findings suggest that GBE is a potential functional food and therapeutic candidate for bone diseases involving osteoclasts.<\/jats:p>","DOI":"10.1002\/jcb.30518","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T10:58:05Z","timestamp":1705316285000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inhibition of receptor activator of nuclear factor kappa\u2010B ligand\u2010mediated osteoclast differentiation and bone resorption by <i>Gryllus bimaculatus<\/i> extract: An in vitro study"],"prefix":"10.1002","volume":"125","author":[{"given":"So Young","family":"Eun","sequence":"first","affiliation":[{"name":"Musculoskeletal and Immune Disease Research Institute, School of Medicine Wonkwang University Iksan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyeong","family":"Do Park","sequence":"additional","affiliation":[{"name":"Musculoskeletal and Immune Disease Research Institute, School of Medicine Wonkwang University Iksan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon\u2010Hee","family":"Cheon","sequence":"additional","affiliation":[{"name":"Musculoskeletal and Immune Disease Research Institute, School of Medicine Wonkwang University Iksan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myeung Su","family":"Lee","sequence":"additional","affiliation":[{"name":"Musculoskeletal and Immune Disease Research Institute, School of Medicine Wonkwang University Iksan South Korea"},{"name":"Division of Rheumatology, Department of Internal Medicine Wonkwang University Hospital Iksan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hae Joong","family":"Cho","sequence":"additional","affiliation":[{"name":"Musculoskeletal and Immune Disease Research Institute, School of Medicine Wonkwang University Iksan South Korea"},{"name":"Department of Obstetrics and Gynecology Wonkwang University Hospital Iksan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4493-0264","authenticated-orcid":false,"given":"Ju\u2010Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Musculoskeletal and Immune Disease Research Institute, School of Medicine Wonkwang University Iksan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092073"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.7326\/AITC201708010"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1513724"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01658"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(11)60752-0"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-008-0825-6"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-018-0040-9"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.160230"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00647"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002483"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1323"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2013.137"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.687712"},{"key":"e_1_2_10_14_1","first-page":"108","volume-title":"Looking at edible insects from a food safety perspective. 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