{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:44:37Z","timestamp":1792115077792,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T00:00:00Z","timestamp":1707782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Strong evidence has indicated that upregulation of chemokine (CC motif) ligand\u20102 (CCL2) expression and the presence of an inflammatory tumor microenvironment significantly contribute to the migratory and invasive properties of oral squamous cell carcinoma, specifically oral tongue squamous cell carcinoma (OTSCC). However, the precise epigenetic mechanism responsible for enhanced CCL2 expression in response to the inflammatory mediator tumor necrosis factor alpha (TNF\u2010\u03b1) in OTSCC remains inadequately elucidated. We have demonstrated that the production of CCL2 can be induced by TNF\u2010\u03b1, and this induction is mediated by the chromatin remodel protein BRG1. Through the use of a chromatin immunoprecipitation (ChIP) assay, we have found that BRG1 was involved in the recruitment of acetylated histones H3 and H4 at the <jats:italic>CCL2<\/jats:italic> promoter, thereby activating TNF\u2010\u03b1\u2010induced CCL2 transcription. Furthermore, we have observed that recruitment of NF\u2010\u03baB p65 to the CCL2 promoter was increased following BRG1 overexpression and decreased after BRG1 knockdown in OTSCC cells. Our Re\u2010ChIP assay has shown that BRG1 knockdown completely inhibits the recruitment of both acetylated histone H3 or H4 and NF\u2010\u03baB to the CCL2 promoter. In summary, the findings of our study demonstrate that BRG1 plays a significant role in mediating the production of CCL2 in OTSCC cells in response to TNF\u2010\u03b1 stimulation. This process involves the cooperative action of acetylated histone and NF\u2010\u03baB recruitment to the CCL2 promoter site. Our data suggest that BRG1 serves as a critical epigenetic mediator in the regulation of TNF\u2010\u03b1\u2010induced CCL2 transcription in OTSCC cells.<\/jats:p>","DOI":"10.1002\/jcb.30535","type":"journal-article","created":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T09:58:42Z","timestamp":1707818322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["BRG1 mediates epigenetic regulation of TNF\u03b1\u2010induced <i>CCL2<\/i> expression in oral tongue squamous cell carcinoma cells"],"prefix":"10.1002","volume":"125","author":[{"given":"Mingyan","family":"Xu","sequence":"first","affiliation":[{"name":"School of Stomatology Fujian Medical University Fuzhou China"},{"name":"Department of Implantology Stomatological Hospital of Xiamen Medical College &amp; Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment Xiamen Fujian 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