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However, it remains poorly characterized and its direct function in cancer remains unclear. The study presented here aims to further examine whether PTGFRN expression affects a cancer cell's phenotype, as well as metastatic\u2010like characteristics. We used stable shRNA and cDNA transfections to respectively knockdown and overexpress PTGFRN in three different cancer cell lines, two of which are representative of rare and aggressive cancers (Mesothelioma and Pediatric Medulloblastoma). We then examined the characteristics of the resulting clones and showed a decrease in proliferation, migration, colony formation, and spheroid growth capabilities in cells where PTGFRN expression had been inhibited, while cells overexpressing PTGFRN showed the opposite. In addition, we showed that PTGFRN displayed direct binding to two protein partners, Integrin \u03b21 and E. Cadherin, the latter of which is a novel direct binding partner to PTGFRN. Furthermore, silencing PTGFRN expression impacted the cellular process of autophagy, thereby providing another avenue by which PTGFRN potentially contributes to a cancer cell phenotype. Our findings demonstrate the potential role of PTGFRN in cancer metastasis and suggest PTGFRN as a future target for drug development in the treatment of metastatic cancers.<\/jats:p>","DOI":"10.1002\/jcb.30616","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T11:43:32Z","timestamp":1719229412000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prostaglandin F2 Receptor Negative Regulator (PTGFRN) Expression Correlates With a Metastatic\u2010like Phenotype in Epidermoid Carcinoma, Pediatric Medulloblastoma, and Mesothelioma"],"prefix":"10.1002","volume":"125","author":[{"given":"Jorge","family":"Marquez","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Baltimore School of Pharmacy University of Maryland Baltimore Maryland USA"},{"name":"Target Discovery Division A&amp;G Pharmaceutical Inc. 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