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This study aims to evaluate CHR's potential to inhibit osteoclastogenesis and prevent bone deterioration in both in vitro and in vivo models. Using tartaric acid\u2010resistant acid phosphatase staining and hydroxyapatite resorption assays, we examined the impact of CHR on RANKL\u2010induced osteoclasts derived from mouse bone marrow monocytes. Additionally, Western blot analysis and qRT\u2010PCR were utilized to assess the protein and gene expressions within the MAPK and NF\u2010\u03baB signaling pathways, as well as the NFATc1 pathway. In vivo, CHR's effects were validated using micro\u2010CT and histomorphometry in an ovariectomized mouse model, showing significant reduction in osteoclast activity and bone loss. The study confirms CHR's inhibition of osteoclastogenesis through interference with RANKL\u2010mediated signaling pathways, suggesting its potential as a novel therapeutic agent for osteolytic conditions related to osteoclast\u2010osteoblast dysregulation.<\/jats:p>","DOI":"10.1002\/jcb.30670","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T13:04:47Z","timestamp":1730466287000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Effects and Mechanisms of Chrysosplenetin in Targeting RANKL\u2010Induced NF\u2010\u03baB Signaling and NFATc1 Activation to Protect Bone Density in Osteolytic Diseases"],"prefix":"10.1002","volume":"126","author":[{"given":"Guoju","family":"Hong","sequence":"first","affiliation":[{"name":"Second Clinical Medical College Guangzhou University of Chinese Medicine Guangzhou Guangdong China"},{"name":"Department of Orthopaedics Guangdong Provincial Hospital of Chinese Medicine Guangzhou Guangdong China"},{"name":"Guangdong Provincial Academy of Chinese Medical Sciences Guangdong Provincial Hospital of Chinese Medicine Guangzhou Guangdong China"},{"name":"Traumatology &amp; Orthopedics Institute Guangzhou University of Chinese Medicine Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University Guangzhou Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[{"name":"Traumatology &amp; Orthopedics Institute Guangzhou University of Chinese Medicine Guangzhou Guangdong China"},{"name":"Department of Orthopaedics The Third Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou Guangdong China"},{"name":"Shenzhen Institute of Advanced Technology Chinese Academy of Science Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushi","family":"Wei","sequence":"additional","affiliation":[{"name":"Traumatology &amp; Orthopedics Institute Guangzhou University of Chinese Medicine Guangzhou Guangdong China"},{"name":"Department of Orthopaedics The Third Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou Guangdong China"},{"name":"Shenzhen Institute of Advanced Technology Chinese Academy of Science Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2021-8309","authenticated-orcid":false,"given":"Jiake","family":"Xu","sequence":"additional","affiliation":[{"name":"Shenzhen Institute of Advanced Technology Chinese Academy of Science Shenzhen Guangdong China"},{"name":"School of Biomedical Sciences The University of Western Australia Perth Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092073"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2019.04.008"},{"key":"e_1_2_13_4_1","first-page":"347","article-title":"Biological Basis of Bone Strength: Anatomy, Physiology and Measurement","volume":"20","author":"Hart N. 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