{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T05:53:04Z","timestamp":1775281984164,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Streptococcus pneumonia<\/jats:italic> is a Gram\u2010positive and facultative anaerobic bacterium that causes a number of diseases, including otitis media, community\u2010acquired pneumonia, sepsis, and meningitis. With the emergence of antibiotic\u2010resistant strains, there is an urgent need to develop antibiotics with a novel mechanism. The toxin\u2013antitoxin (TA) system, which is primarily found in prokaryotes, consists of a toxin and its equivalent antitoxin genes. The YefM\u2013YoeB module is a Type II TA system, where the YoeB toxin functions as a putative mRNA interferase upon activation, while the YefM antitoxin acts as a transcription repressor by binding to its promoter region along with YoeB. In this study, we determined the crystal structure of the YefM\u2013YoeB complex from <jats:italic>S<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> TIGR4 to comprehend the binding mechanism of the TA system. Furthermore, an in vitro ribonuclease activity assay was conducted to identify the ribonuclease activity of the YoeB toxin. Additionally, furthermore, the oligomeric state of the YefM\u2013YoeB complex in solution was investigated, and a DNA\u2010binding mode was proposed. These structural and functional insights into the YefM\u2013YoeB complex could provide valuable information for the development of novel antibiotics targeting <jats:italic>S<\/jats:italic>. <jats:italic>pneumonia<\/jats:italic>\u2010associated diseases.<\/jats:p>","DOI":"10.1002\/jcb.30672","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T11:09:59Z","timestamp":1731409799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Structural and Functional Insight Into YefM\u2013YoeB Complex of Toxin\u2013Antitoxin System From <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1002","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2864-9891","authenticated-orcid":false,"given":"Do\u2010Hee","family":"Kim","sequence":"first","affiliation":[{"name":"Research Institute of Pharmaceutical Sciences College of Pharmacy, Sookmyung Women's University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010Chan","family":"Lee","sequence":"additional","affiliation":[{"name":"The Research Institute of Pharmaceutical Sciences College of Pharmacy, Seoul National University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenglong","family":"Jin","sequence":"additional","affiliation":[{"name":"The Research Institute of Pharmaceutical Sciences College of Pharmacy, Seoul National University Seoul Republic of Korea"},{"name":"Mastermeditech Ltd. Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung\u2010Min","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Duksung Women's University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Jin","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Dongduk Women's University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoon\u2010Seok","family":"Kang","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Program in Advanced Convergence Technology &amp; Science Jeju National University Jeju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bong\u2010Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Mastermeditech Ltd. Seoul Republic of Korea"},{"name":"College of Pharmacy Ajou University Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2022.127221"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuaa072"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9050420"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00058-15"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00661-1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2022.127159"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00763-19"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1033"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-33253-1_18"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10090378"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00030-12"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1028"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa706"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319011471"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2013.02.008"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/355472a0"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_9_21_1","unstructured":"\u201cThe PyMOL Molecular Graphics System \u201d Version 2.5 Schr\u00f6dinger (LLC)."},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.729513"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.05.022"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt742"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4519"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.07.004"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00959"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05187-11"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.101457"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.30672","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T10:22:20Z","timestamp":1745835740000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.30672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,12]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/jcb.30672"],"URL":"https:\/\/doi.org\/10.1002\/jcb.30672","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,12]]},"assertion":[{"value":"2024-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e30672"}}