{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:30:01Z","timestamp":1789583401487,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Breast cancer ranks among the most prevalent cancers. Enhancing the effectiveness of chemotherapy and patient survival is the objective of many studies. In the literature, no study has investigated the combined effect of vitamin c and curcumin with chemotherapy drugs on cell viability in the MCF\u20107 cell line, nor the mechanism of inflammation induced by cancer drugs, both in vitro and in silico. Thus, the purpose of this study was to assess the synergistic effect of curcumin and vitamin c in combination with the chemotherapy drugs 5\u2010fluorouracil and doxorubicin. The cytokine hub genes of the Toll\u2010like receptor pathway for the administered drugs were identified using the Cytoscape program, and docking studies were conducted via the Cb Dock2 website. In silico analyses indicated that doxorubicin and curcumin displayed comparable characteristics, achieving the highest interaction scores (\u201010) with marker proteins, whereas 5\u2010fluorouracil and vitamin c showed lower interaction scores. Cell viability was evaluated through MTT analysis and AO\/PI staining, while the expression of inflammation\u2010related markers IL\u20106, IL\u201010, and TNF\u2010\u03b1 proteins determined using the ELISA method. After 24 h, the cell viability of the chemotherapeutic drugs administered in combination with curcumin decreased by up to 28%. Subsequently, applications at 48 and 72\u2009h were performed. These results indicate that the effect of curcumin on cell viability is significant when combined with chemotherapy drugs. In the ELISA test, a 52% expression of IL\u20106 was noted in MCF\u20107 cells treated with curcumin, whereas the IL\u20106 level decreased to 15% in the other experimental groups. An increase was observed in the TNF\u2010\u03b1 expression with 5\u2010fluorouracil and doxorubicin compared to the control, while a notable decrease was recorded in the applications with vitamin c and curcumin (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). This study demonstrates that vitamin c and curcumin exhibit a synergistic effect with chemotherapeutic agents in the inflammatory system.\n                  <\/jats:p>","DOI":"10.1002\/jcb.30688","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T02:57:00Z","timestamp":1734577020000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In Silico and In Vitro Verification of the Effects of Chemotherapeutic Doxorubicin and 5\u2010Fluorouracil in Combination With Curcumin and Vitamin C on MCF\u20107 Cells"],"prefix":"10.1002","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0552-1051","authenticated-orcid":false,"given":"Asl\u0131","family":"Aky\u00fcz","sequence":"first","affiliation":[{"name":"Department of Biology Tekirdag Namik Kemal University Tekirdag T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1224-442X","authenticated-orcid":false,"given":"Duygu YA\u015eAR","family":"\u015eirin","sequence":"additional","affiliation":[{"name":"Department of Biology Tekirdag Namik Kemal University Tekirdag T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"A.Karaku\u015f \u201cEffect of AKT Inhibitor on Apoptosis in Human Breast Cancer Cell Line (MCF\u20107) \u201d (doctoral thesis Atat\u00fcrk University Department of Science 2022)."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13193-016-0512-6"},{"key":"e_1_2_9_4_1","unstructured":"Z.Azizi \u201cInvestigation of the Relationship Between NEAT1 Expression Level and Doxorubicin Resistance in Breast Cancer Cell Lines \u201d (master's thesis Pamukkale University Institute of Health Sciences Denizli 2022)."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/62120"},{"key":"e_1_2_9_6_1","unstructured":"N.Farnoud \u201cEffects of Routine Flavonoid on the Toxicity and Antitumor Activities of Oxaliplatin and 5\u2010Fluorouracil in CACO2 Cancer Cells \u201d (doctoral thesis Ankara University Institute of Health Sciences Department of Biochemistry 2015)."},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/354614"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24142527"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods6100092"},{"key":"e_1_2_9_10_1","unstructured":"M.Akpolat \u201cInvestigation of the Protective Effects of Curcumin and Vitamin C Against the Damage Caused by Gamma Radiation on Ileum Goblet Cells at Light and Electron Microscopic Levels \u201d (doctoral thesis Trakya University Institute of Health Sciences Edirne 2007)."},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/jcrt.JCRT_989_17"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10121894"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031809"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-018-0221-4"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules200713384"},{"key":"e_1_2_9_16_1","unstructured":"C.\u2010P.SegeritzandV.Ludovic Cell Culture: Growing Cells as Model SystemsIn Vitro(Cambridge United Kingdom: 2 Wellcome Trust Sanger Institute Hinxton United Kingdom 2017)."},{"key":"e_1_2_9_17_1","unstructured":"H.Vatansever Basic Principles of Cell Culture and Introduction of the Laboratory 2016."},{"key":"e_1_2_9_18_1","unstructured":"L. R.TerryandA.Richard Assay Guidance Manual. Eli Lilly & Company and the National Center for Advancing Translational Sciences."},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.11-4-397"},{"key":"e_1_2_9_20_1","unstructured":"D.Demirba\u015f \u201cEvaluation of the Relationship Between Cytokine (tumor necrosis Factor Alpha Transforming Growth Factor Beta\u20101 Interferon Gamma Interleukin\u201010) Gene Polymorphisms and the Role of Gene Expressions In Etiopathogenesis With Clinical Parameters in Hodgkin Lymphoma Patients \u201d (specialization thesis Gaziantep University Faculty of Medicine 2020)."},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20030407"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.30688","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T06:22:41Z","timestamp":1745821361000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.30688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,18]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/jcb.30688"],"URL":"https:\/\/doi.org\/10.1002\/jcb.30688","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,18]]},"assertion":[{"value":"2024-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e30688"}}