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Emerging evidence highlights the involvement of tumor cell\u2010derived exosomes in tumor\u2010associated microglia polarization via transfer of exosomal onco\u2010proteins and miRNAs. Although the regulatory role of long noncoding RNAs (lncRNAs) in immune signaling are known, its mechanism in microglial polarization via exosomes in GBM still remains poorly understood. In our study, we found that in comparison to the normoxic GBM\u2010derived exosomes lncRNA H19 was significantly upregulated in hypoxic GBM\u2010derived exosomes. Hypoxic GBM\u2010derived exosomes and secretome (conditioned media) caused the reduction in the % phagocytosis of microglia as compared with the control group. Moreover, GBM secretome caused increase in the M2\u2010specific genes (IL10, STAT\u20103, CD163, CD206) in microglia indicating its polarization to the protumorigenic (M2) phenotype. LncRNA H19 knocked down GBM\u2010secretome treatment in microglia further reduced the STAT\u20103 expression indicating H19 mediated signaling. Overall, our results suggest the involvement of hypoxic exosomes and lncRNA H19 in microglial polarization and H19 as a potential target.<\/jats:p>","DOI":"10.1002\/jcb.70002","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T07:34:25Z","timestamp":1738740865000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hypoxic Secretome and Exosomes Derived From Human Glioblastoma Cells (U87MG) Promote Protumorigenic Phenotype of Microglia in Vitro"],"prefix":"10.1002","volume":"126","author":[{"given":"Sangati","family":"Pancholi","sequence":"first","affiliation":[{"name":"Department of Zoology, Division of Neurobiology, Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritvi","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Zoology, Division of Neurobiology, 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