{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:51:07Z","timestamp":1792000267184,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Paracellular permeability of mineral ions and water in the intestines is restricted by claudins (CLDNs), which are key components of tight junctions (TJs). Glutamate (Glu) plays a role in regulating absorption mechanisms in the intestines, but the effect on paracellular permeability remains unclear. In mouse intestine\u2010derived MCE301 cells, Glu treatment increased the mRNA levels of\n                    <jats:italic>CLDN10b<\/jats:italic>\n                    , while the levels of other CLDNs and zonula occludens\u20101, a scaffolding protein in TJs, remained unchanged. Similar results were obtained in rat intestine\u2010derived IEC6 cells without CLDN1 and CLDN15. Glu also increased the protein level and TJ localization of CLDN10. The Glu\u2010induced increase in\n                    <jats:italic>CLDN10b<\/jats:italic>\n                    mRNA expression was suppressed by knockdown of taste receptor type 1, T1R1 and T1R3. Glu treatment stimulated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx, which was inhibited by BAPTA, a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    chelator, and ebselen, a voltage\u2010dependent Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel inhibitor. Furthermore, the upregulation of\n                    <jats:italic>CLDN10b<\/jats:italic>\n                    mRNA by Glu was inhibited by BAPTA, ebselen, and rapamycin, an inhibitor of mammalian target of rapamycin (mTOR). These results suggest that Glu upregulates CLDN10b expression in MCE301 cells mediated by a T1R1\/T1R3\u2010Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010mTOR signaling pathway. The mRNA levels of\n                    <jats:italic>CLDN10b<\/jats:italic>\n                    were decreased in the colon of aged mice and MCE301 cells treated with tenovin\u20101 (Ten\u20101), an inducer of cellular senescence. Glu reversed the Ten\u20101\u2010induced reduction of\n                    <jats:italic>CLDN10b<\/jats:italic>\n                    mRNA and restored paracellular barrier function. These results suggest that Glu enhances the intestinal paracellular barrier function through the regulation of CLDN10b expression.\n                  <\/jats:p>","DOI":"10.1002\/jcb.70077","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T09:58:17Z","timestamp":1764151097000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rescue of Aging\u2010Dependent Reduction of Claudin\u201010b Expression by Glutamate in Mouse Intestinal MCE301 Cells"],"prefix":"10.1002","volume":"126","author":[{"given":"Miki","family":"Tanabe","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry, Department of Biopharmaceutical Sciences Gifu Pharmaceutical University Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Biopharmaceutical Sciences 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